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Agronomía Mesoamericana

On-line version ISSN 2215-3608Print version ISSN 1659-1321


OSORIO-GIRALDO, Inés; GUTIERREZ-SANCHEZ, Pablo  and  MARIN-MONTOYA, Mauricio. Genetic variability of colombian isolates of  Potato mop-top virus (PMTV). Agron. Mesoam [online]. 2013, vol.24, n.1, pp.01-15. ISSN 2215-3608.

The objective of  this work was to  study  the  level  of  variation  of  PMTV  isolates  from Colombian  potato  crops  through  2010  and  2011.  Genes encoding for the  viral coat protein (CP, ARN 2) and the Triple  Gene Block (TGB2, ARN 3) were sequenced from PMTV  isolates  obtained  in  the  provinces  of  Antioquia, Boyacá,  Cundinamarca  y  Nariño.  Additionally,  RNA  2 and RNA 3 from two  isolates  were almost sequenced to completion  (>83%).  Phylogenetic  analysis  based  on  the CP  sequence  revealed  the  presences  of  two  clades.  The first clade included PMTV reference strains  from all over the world and 19 Colombian isolates  used in this study. The  second  class  grouped  only  Colombian  isolates  and shared less than 76%  identity with clade 1, suggesting a new pomovirus  species. Complete genome sequencing is required  to  confirm this hypothesis. Phylogenetic analysis using the TGB2 gene grouped all sequences in a single clade. Sequence analysis of segments 2 and 3 of the viral genomes revealed >94% sequence identity with PMTV isolates from Czech Republic and Sweden. These findings will be helpful in the development of  diagnostic tools for PMTV in the Andes in order to support tuber-seed certification and genetic improvement programs.

Keywords : RT-PCR; sequencing; Solanum tuberosum.

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