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Agronomía Mesoamericana

On-line version ISSN 2215-3608Print version ISSN 1659-1321


CALVO-ARAYA, José Alonso; RIVERA-COTO, Germán; OROZCO-CAYASSO, Steffany  and  OROZCO-RODRIGUEZ, Rafael. Isolation  and in vitro evaluation of  antagonists to Botrytis cinerea in blackberry. Agron. Mesoam [online]. 2012, vol.23, n.2, pp.225-231. ISSN 2215-3608.

The aim of this study was to determine the antagonistic capacity of fungi to B. cinerea in blackberry crops in Costa Rica. During the first semester of 2009, 35  isolates of native filamentous fungi from the carpoplane of blackberry fruits were obtained, six of which belonging to the genus Trichoderma sp.  were selected to evaluate their antagonistic effect in vitro against B. cinerea through  the  dual-culture   technique.  In  this  evaluation, competition for the substrate and the antibiotic effect were determined. The first one was calculated using Bell’s scale, and for the assessment of antibiotic effect, percent inhibition of radial growth (PIRG) was calculated.  All tested isolates effectively competed for substrate against B. cinerea, within Trichoderma  strains  Lu13  and  Lu15  excelled,  achieving grade I in the scale  used, where the antagonist overgrew the pathogen  covering 100% of the culture medium. The remaining isolates of Trichoderma, were classified in grade II of  antagonism. As  to  the  antibiotic  effect,  all  isolates inhibited  mycelia  growth  of  B.  cinerea,  four  of  which showed an effectiveness  higher than 80% in this in vitro screening technique.

Keywords : gray mold; antagonistic test; Trichoderma; antibiosis.

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