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On-line version ISSN 1659-097X


LOZADA MEDINA, Jesús León; PADILLA ALVARADO, José Rafael  and  CORTINA NUNEZ, Manuel de Jesús. Biochemical and Physiological Responses in Sedentary Subjects During the Evaluation of Maximum Oxygen Uptake. MHSalud [online]. 2023, vol.20, n.1, pp.113-126. ISSN 1659-097X.


The use of incremental exercise tests (IET) to evaluate the individual’s acute responses is an essential tool in Physical Activity Science.


This paper aims to analyze the behavior between biochemical and physiological variables as aerobic-anaerobic transition indicators during two incremental exercise tests, which measure the maximum oxygen uptake in healthy subjects.


The sample consisted of two individuals, who were thirty-three and twenty-five years old, respectively. During the execution of the tests, the following materials and tools were used: a portable glucometer, a portable lactometer, reactive tapes, a treadmill, a stationary Cycle Ergometer Monark®, a heart rate monitor Polar®, a gas analyzer, software Breeze®, a computer, tools for recording data, and a pencil.


the high inverse correlation between the Glycemic index and VO2 (r: -0,853) and the Glycemic and CO2 (r: -0,851) are notable. Moreover, the detection of thresholds for each subject through blood lactate invasive and non-invasive methods, such as heart rate (HR), is also shown.


A clear explanation of Respiratory Quotient (RQ) behavior is given during the Cycle-ergometer test, concluding that this type of protocol is safe for the group under study and that the validity of the results is in accordance with theoretical expectations.

Keywords : lactic acid; aerobic exercise; heart rate; blood glucose.

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