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Revista Costarricense de Salud Pública

Print version ISSN 1409-1429

Abstract

LERSY, López G; ALFONSO  and  SUAREZ M, Héctor. Microbiology and Molecular Characterization of Staphylococcus aureus in meat products marketed in Cartagena, Colombia. Rev. costarric. salud pública [online]. 2016, vol.25, n.2, pp.81-89. ISSN 1409-1429.

Staphylococcus aureus at present not only is part of nosocomial microorganisms but also of food - borne diseases.

Objetive:

Quantify and characterize by conventional microbiological and molecular analysis methodology to Staphylococcus in beef and pork meat.

Method:

They were used 160 samples of ground beef and pork chop, 40 outlets in three locations in the city of Cartagena: 1. Historic North and Caribbean (LH) 2. Bay Industrial (LI) 3. Virgin and Tourism (LV). For microbiological identification was used the plate count technique and the technique of Polymerase Chain Reaction (PCR).

Results:

The conventional microbiological methods Staphylococcus aureus was identified in 100 % of samples, while the PCR confirmed the presence of Staphylococcus aureus in 88 %. In 72 % of outlets were found counts > 100 CFU/g, 27 % had counts < 100 CFU/g. The highest percentage was found in the industrial town of Bay (48,2 %), followed by the Historical town and North Caribbean with 34,4 %. 32,5 % of analyzed are marketing outlets and ground pork chop unfit for human consumption meat, to present counts that exceed benchmarks for Staphylococcus aureus in raw meat chopped and ground (100-1000 CFU/g). It was determined the presence of the MecA gene encoding strains of methicillin-resistant Staphylococcus aureus (MRSA) in two samples by molecular methods is not for Staphylococcus aureus.

Keywords : food safety; meat; food; bacteria.

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