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Revista Costarricense de Salud Pública

Print version ISSN 1409-1429


MADRIGAL, Ana Patricia et al. Bacteriological study of prepared and commercialized sushi in San Jose, Costa Rica. Rev. costarric. salud pública [online]. 2013, vol.22, n.1, pp.51-55. ISSN 1409-1429.

Objective: Knowing the bacteriological quality of samples issued at different sushi restaurants in the province of San José in Costa Rica. Methods: Sixty sushi samples, acquired from different restaurants from the province of San José, Costa Rica were analyzed in order to determine their bacteriological quality. Methodology followed was that described in the Compendium of Methods for the Microbiological Examination of Food and included total aerobic plate count, Staphylococus aureus plate count, Most Probable Number of total and fecal coliforms, Escherichia coli, and B. cereus. Also, the presence of potential pathogens found in sushi including Salmonella sp., Listeria monocytogenes and Vibrio parahaemolyticus was analyzed. Results: That 6 (46%) of the restaurants were positive for E. coli, even though in small quantities (1 to to NMP/g average). S. aureus was isolated from seven restaurants (54%) also in small concentrations (102CFU/g). In addition, two strains of B cereus and two of L monocytogenes were isolated. Neither Salmonella sp nor Vibrio parahaemolyticus were isolated from the samples evaluated. Discussion: According to the Central American Technical Regulation RTCA 67 04 50 08 sections 17.1 62% (8) of the restaurants included in this study do not comply with the regulations established for this kind of food. This nonconformity is due, mainly, to the presence of S aureus in concentrations greater than 100 CFU/g.

Keywords : Coliforms; Escherichia coli; Staphylococcus aureus; Listeria monocytogenes; B. cereus.

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