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Agronomía Costarricense

Print version ISSN 0377-9424


AMADOR, Marianela et al. Use of the entomopathogenic nematode Heterorhabditis atacamensis CIA- NE07 in the control of banana weevil Cosmopolites sordidus in vitro. Agron. Costarricense [online]. 2015, vol.39, suppl.1, pp.47-60. ISSN 0377-9424.

Among the species of banana borers, black weevil (Cosmopolites sordidus) is the most economically important pest in Costa Rica and worldwide. The control of C. sordidus in intensive production systems is mainly based on application of insecticides; therefore the search for biological alternatives, such as the use of entomopathogenic nematodes (EPN), is needed. The susceptibility of Cosmopolites sordidus to Heterorhabditis atacamensis CIANE07 was evaluated. The effect of inoculating H. atacamensis on larvae and adults of C. sordidus, in vitro and in artificially infected corms, was evaluated. Larvae inoculated with EPN had a mortality of 88% on the second day and 100% on the third day; no mortality was observed in adults. The treatments of 100, 500 and 1000 IJ.larvae-1 showed statistically significant differences from the control and theLD50 was 52 IJ.larvae-1. When the larvae were placed within the corms the LD50 increased to 375 IJ.larvae-1. The results indicate that the strain H. atacamensis CIA-NE07 is capable of locating and infecting weevil larvae within the banana corm and reach infection levels over 80%, 10 days after inoculation at doses of 1000 and 2000 IJ.larvae-1. The entomopathogenic nematodes are a viable alternative to be considered in the Integrated Pest Management programs of black weevil, in crops such us banana and plantain.

Keywords : Entomopathogenic nematodes; Cosmopolites sordidus; Heterorhabditis; biological control.

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