SciELO - Scientific Electronic Library Online

vol.37 issue1Identification of the agent causing anthracnose on Sansevieria spp., in Costa RicaGenetic resistance of tomato hybrids (Solanum lycopersicum L. (Mill.) to tomato spotted wilt virus (TSWV) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • Have no similar articlesSimilars in SciELO


Agronomía Costarricense

Print version ISSN 0377-9424


GOMEZ, Ana; VARGAS CASTILLO, Pilar  and  ABDELNOUR ESQUIVEL, Ana. Cryopreservation of teak (Tectona grandis L.f) seeds. Agron. Costarricense [online]. 2013, vol.37, n.1, pp.51-60. ISSN 0377-9424.

Teak (Tectona grandis L.f) is a tropical tree of commercial value due to the high demand of its high-quality wood and rapid growth. Genetic improvement programs for this species have resulted in seeds of better quality and, at the same time, improvement in the quality of plantations. To preserve genetic diversity and guarantee the raw material for improvement programs and for future reproduction, seeds are kept under conventional seed banks conditions with temperature ranging between 4 and -20ºC. However, there are other means to conserve this valuable germplasm. Cryopreservation is the storage of plant material in liquid nitrogen (NL, -196ºC) and its major advantage is the conservation for indefinite periods of time, under high genetic stability conditions. Survival and regeneration of plants after seed freezing in liquid nitrogen (LN) were evaluated in this work, using the dessiccation and rapid freezing technique. The methodology was tested both, in seeds isolated from endocarps (seeds) asin seeds inside endocarps (seeds with endocarps), with germination rates, after thawing and 28 days in culture, of 84% and 70% respectively.

Keywords : Cryopreservation; seed; endocarp; teak; Tectona grandis; liquid nitrogen.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License