SciELO - Scientific Electronic Library Online

vol.70 issue1Predation, reproduction, and development of the lady beetle Hippodamia convergens (Coleoptera: Coccinellidae)In vitro culture of hairy roots of the shrub, Phyllanthus acuminatus (Phyllanthaceae) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista de Biología Tropical

On-line version ISSN 0034-7744Print version ISSN 0034-7744


OLMEDO, Camila; KOCH, Alma; SARMIENTO, Berenice  and  IZQUIERDO, Andrés. Antifungal potential of biosurfactants produced by strains of Bacillus spp. (Bacillales: Bacillaceae) selected by molecular screening. Rev. biol. trop [online]. 2022, vol.70, n.1, pp.636-646. ISSN 0034-7744.


Bacillus species are used as biological controllers for phytopathogenic fungi, and the mechanisms to produce controllers include biosynthesis of lipopeptide biosurfactants with antifungal activity.


To evaluate the antifungal potential of the biosurfactants produced by Bacillus strains, selected by molecular screening, on Fusarium oxysporum.


We selected four molecular markers, related to the biosynthesis of surfactin, fengicin, and lichenysin (srfA, spf, fenB, LichAA) in nine Bacillus strains. We used two mineral media with several culture conditions, for biosurfactant production, and a well diffusion test for antifungal potential.


Only the biosurfactant produced by UFAB25 inhibits the mycelial growth of F. oxysporum (44 % ± 13): this biosurfactant was positive for srfA, spf, and fenB genes involved in the synthesis of surfactin and fengicine. Antifungal activity depends on culture conditions and the strain.


Genetic markers are useful to detect strains with antifungal potential, facilitating the selection of bio-controllers. The biosurfactant profile is influenced by the strain and by culture conditions.

Keywords : microbial surfactant; native strains; Bacillus subtilis; antifungal activity; molecular marker..

        · abstract in Spanish     · text in English     · English ( pdf )