SciELO - Scientific Electronic Library Online

vol.68 issue1Floristic diversity of the tropical dry forest in the lower and middle Sinú subregion, Córdoba, ColombiaAnatomy of the visceral cavity of Eugerresmexicanus (Teleostei: Gerreidae) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista de Biología Tropical

On-line version ISSN 0034-7744Print version ISSN 0034-7744


MEI, Zhiqiang; KHAN, Md.-Asaduzzaman  and  FU, Junjiang. Genetic authentication of Eclipta prostrate (Asteraceae) from Penthorum chinense (Penthoraceae) by Sequence Characterized Amplified Region (SCAR) markers. Rev. biol. trop [online]. 2020, vol.68, n.1, pp.180-188. ISSN 0034-7744.


For the rapid and accurate genetic identification and authentication of living organisms, improved random amplified polymorphic DNA (RAPD) fragment based development of sequence-characterized amplified region (SCAR) markers is an important genetic technique.


This study aimed to develop SCAR markers for perennial herb Eclipta prostrate (E. prostrate).


Here the RAPD fragments by improved RAPD amplification with primers A11 and N-7 for E. prostrate were cloned into pGEX-T vector, and PCR amplification identified the positive clones. After the enzymatic digestion, they were sequenced with Sanger sequencing.


Two SCAR markers were developed, which were very specific to E. prostrate, not found in Penthorum chinense Pursh(P. chinense). The nucleotide sequence search by BLAST GenBank database showed that they are novel in E. prostrate, therefore they were deposited in Genbank with accession number KX671034, KX671035. The markers did not show any identity to other species.


Thus, in this study two specific SCAR markers were developed for genetically distinguishing and identifying the plant species E. prostrate from herb P. chinense and others.

Keywords : Eclipta prostrate; genetic authentication; Penthorum chinense Pursh; random amplified polymorphic DNA; sequence characterized amplified region.

        · abstract in Spanish     · text in English     · English ( pdf )