SciELO - Scientific Electronic Library Online

vol.54 issue3Actividad ovárica del tepezcuintle Agouti paca (Rodentia: Agoutidae) en cautiverioScarlet Macaw, Ara macao, (Psittaciformes: Psittacidae) diet in Central Pacific Costa Rica author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista de Biología Tropical

On-line version ISSN 0034-7744Print version ISSN 0034-7744


MONTES-PEREZ, Rubén C; ECHEVERRIA GARCIA, Adán W; ZAVALA CASTRO, Jorge  and  ALFARO GAMBOA, Militza G. Variaciones nucleotídicas de dos grupos de tepezcuintles, Agouti paca (Rodentia: Agoutidae), en cautiverio provenientes de dos localidades de Yucatán, México. Rev. biol. trop [online]. 2006, vol.54, n.3, pp.911-917. ISSN 0034-7744.

Nucleotidic variations of two captive groups of tepezcuintle, Agouti paca (Rodentia: Agoutidae), from two sites in Yucatan, Mexico. The objective of this work was to estimate the nucleotidic variation between two groups of tepezcuintles (Agouti paca) from the states of Campeche and Quintana Roo, Mexico and within members of each group. Blood samples were collected from eleven A. paca kept in captivity. DNA from leukocytic cells was used for Ramdom Amplification of DNA Polimorphism (RAPD). The primers three 5’-d(GTAGACCCGT)- 3’ and six 5’-d(CCCGTCAGCA)- 3’ were selected from de Amersham kit (Ready.To.Go. RAPD Analysis Beads, Amersham Pharmacia Biotech), because they produced an adequate number of bands. The electrophoretic pattern of bands obtained was analyzed using software for phylogenetic analysis based on the UPGMA method, to estimate the units of nucleotidic variation. The phylogenetic tree obtained with primer three reveals a dicotomic grouping between the animals from both states in the Yucatan Peninsula showing a divergent value of 1.983 nucleotides per hundred. Animals from Quintana Roo show a grouping with primer six; an additional grouping was observed with animals from Campeche. Nucleotidic variation between both groups was 2.118 nucleotides per hundred. The nucleotidic variation for the two primers within the groups from both states, showed fluctuating values from 0.46 to 1.68 nucleotides per hundred, which indicates that nucleotidic variation between the two groups of animals is around two nucleotides per hundred and, within the groups, less than 1.7 nucleotides per hundred. Rev. Biol. Trop. 54 (3): 911-917. Epub 2006 Sept. 29.

Keywords : tepezcuintle; Agouti paca; RAPD; nucleotidical variation; phylogenetic tree; nucleotides.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License