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Revista de Biología Tropical

On-line version ISSN 0034-7744Print version ISSN 0034-7744


CHAVES, Fernando; LEON, Guillermo  and  HERNANDEZ-CHAVARRIA, Francisco. Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones. Rev. biol. trop [online]. 2006, vol.54, n.2, pp.253-256. ISSN 0034-7744.

Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays. Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice. Rev. Biol. Trop. 54(2): 253- 256. Epub 2006 Jun 01.

Keywords : Clostridium tetani; tetanus toxin; enzyme-immunoassay; biological assay.

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