SciELO - Scientific Electronic Library Online

vol.60 issue1Current situation of chronic myeloid leukemia in Costa RicaPrevalence of gastroschisis and omphalocele in Costa Rica author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • Have no similar articlesSimilars in SciELO


Acta Médica Costarricense

On-line version ISSN 0001-6002Print version ISSN 0001-6012


ZELEDON-SANCHEZ, Fernando et al. Healing rate assessment of an acute ulcer in Sprague-Dawley rats by applying autologous mesenchymal stem cells drawn from adipose tissue. Acta méd. costarric [online]. 2018, vol.60, n.1, pp.27-34. ISSN 0001-6002.


To evaluate the rate of cutaneous cicatrization of acute ulcers in Sprague-Dawley rats, after receiving the administration of mesenchymal stem cells derived from adipose tissue.


There were three experimental groups. After an acute ulcer was performed in the backs of the rats they received either stem cells (SC), Clostebol, (NeobolR group, N) or no treatment (control group, C), the ulcer cutaneous healing rate was assessed as follows: (ulcer day 1 - ulcer day X)/in all groups day 1. Stem cells were extracted from adipose tissue in the inguinal pad and then injected in the Stem cells group.


An average of 1.22 ± 0,46 grams (g) of adipose tissue was extracted and 3.5 x 105 cells were isolated with an average 2.4 x 104 inoculant, 95,5% cell viability. The CD29 antigen positivity on the stem cells assessed by flow cytometry was 96,5%. The histological analysis performed 7 days after the clinical healing showed that the SC group showed the highest vascularization and epithelial tissue formation. When comparing the average healing percentage among groups, only the SC group showed significant differences in contrast with the N group (H (1) = 5.61; p < 0,01) and C (H (1) = 10.47; p< 0.001).


This study suggests that mesenchymal cells derived from adipose tissue increase the healing rate.

Keywords : Adipose derived stem cells; ulcer; healing rate; adipose tissue.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )