Abstract

ZUMBADO-SALAS, Greivin et al. Peripheral blood stem cell collection by apheresis. Acta méd. costarric [online]. 2014, vol.56, n.2, pp.65-70. ISSN 0001-6002.

Background: To evaluate performance and quality of peripheral blood stem cell collections performed at the Méxic o Hospital from June 2010 to June 2012, since there is an established minimum dose of CD34⁺ cells that determines the probability of success of engraftment. Methods: A retrospective study using patient records and data obtained from peripheral blood stem cell apheresis procedures carried out at the Haematology Unit of the México Hospital. This information was used to calculate performance and quality values of collections, and the effect of these on the recovery time of haematological parameters in patients transplanted between June 2010 and June 2012. Results: A total of 52 peripheral blood stem cell apheresis procedures were performed to 26 patients (Hodgkin lymphoma 46%, non-Hodgkin lymphoma and multiple myeloma 27%). An average of 8 blood volumes per patient was processed, the median collection was 8.48 x10⁸ mononuclear cells/Kg, of which an average of 3.06 x10⁶/Kg were CD34⁺ cells. The average recovery time of haematological parameters after transplant was 15.05 days for leukocytes and 17.15 days for platelets. An inverse correlation was observed between CD34⁺ infused cell count and time for platelet count recovery, but not for leukocyte count recovery. Conclusions: Cell counts obtained from apheresis collections and recovery time of haematological parameters after transplant were consistent with results described by the medical literature, showing that the protocol followed has been successful. Only leukocyte count previous to apheresis has proved to be useful to predict a quality collection, and CD34⁺ infused cell count is the best parameter to predict a successful engraftment.

Keywords : stem cells; CD34⁺ cells; apheresis; collection.

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