Abstract

SINCHE ARIAS, Danny Fabricio; RAMIREZ PENAHERRERA, Valeria Estefanía  and  VELALCAZAR RHEA, Cristhian Mauricio. Simultaneous saccharification and fermentation of hydrothermally pretreated Jatropha curcas shell for bioethanol production. Ingeniería [online]. 2022, vol.32, n.1, pp.19-32. ISSN 2215-2652.  http://dx.doi.org/10.15517/ri.v32i1.46884.

Biomass from Jatropha curcas has been studied as a raw material for obtaining renewable energy, due to its non-food crop characteristics. One of the most promising processes to produce ethanol from lignocellulosic biomass is simultaneous saccharification and fermentation (SSF). In other studies carried out through SSF experiments, the conversion of glucan to ethanol from Jatropha shell pretreated with acid or alkaline medium was evaluated but not through hydrothermal processes. In the present study, the Jatropha curcas shell was hydrothermally pretreated with liquid hot water at 200 °C for 15 min, and tested in SSF experiments with an enzyme cocktail (Cellic CTec2) and commercial yeast (Saccharomyces cerevisiae). Different enzyme loadings, sterile media and sizes test were used to assess their impact on ethanol yield. The results showed that the maximum ethanol yield (0.160 - 0.177 g ethanol / g dry biomass) and the conversion of cellulose (57.71 - 63.90 %) were achieved with 5 % w/w of solids, 15 FPU/g dry biomass, and 1 mg/ml of yeast. Through this research, an alternative is proposed for integral using of the Jatropha curcas crop for a circular economy.

Keywords : Bioenergy; bioethanol; Jatropha curcas shell; liquid hot water pretreatment; simultaneous saccharification and fermentation.

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