Abstract

CASTRO-PIEDRA, Silvia  and  MORALES-SANCHEZ, Johan. Aislamiento de células madre mesenquimales derivadas de tejido adiposo del pliegue inguinal murino: un protocolo para obtener lo mejor de pequeñas muestras. Tecnología en Marcha [online]. 2019, vol.32, n.4, pp.69-80. ISSN 0379-3982.  http://dx.doi.org/10.18845/tm.v32i4.4793.

Adipose-derived stem cells are being investigated for tissue repair because of their multidifferentiation potential and secreted growth factors as well as cytokines. Currently, there is a lot of preclinical and clinical studies that use these cells to determine the clinical effect in skin wounds, spinal cord injuries, myocardial infarction among others with promising results. In this study, a small sample of adipose tissue was extracted from mice inguinal pad and the stromal vascular fraction was obtained. Then, it was cultivated in vitro in order to promote ASC adhesion and expansion. Growth kinetics, stem cell markers expression and differentiation potential were analyzed. The isolated stem cells showed similar growth kinetics as reported in literature. Thirty percent of analyzed cells were positive for the stem cell markers and they were able to differentiate in adipogenic, chondrogenic and osteogenic lineages. The protocol proven in this work allowed to isolate adipose-derived stem cells from a very small sample that met almost all the criteria established by the International Society for Cell and Gene Therapy, giving rise to future research for stablish this tissue as an excellent source of stem cells and the development of alternative therapies to the treatment of skin wounds.

Keywords : Mesenchymal stem cell; adipose-derived stem cells; regeneration; scaffold; differentiation.

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