Abstract

SANCHEZ-ZUNIGA, Katherine et al. Cytotoxic evaluation of properties in guava fruit extract (Psidium guajava Var. Tai-Kuo-Bar). Tecnología en Marcha [online]. 2017, vol.30, n.4, pp.150-156. ISSN 0379-3982.  http://dx.doi.org/10.18845/tm.v30i4.3424.

[29]

Taiwanese guava was introduced in Costa Rica in 1978 and then has been extensively studied for its medicinal and nutritional properties. Due to its high content of antioxidants, it has been studied in different countries as a potential anticancer agent. In this work we evaluated the cytotoxicity of extracts of pulp and guava peel in two carcinogenic cell lines: lung (H460), and human colorectal adenocarcinoma (SW480), ATCC. Cells were treated at increasing concentrations of 250 to 450 μg / mL with both types of extract for 24, 48 and 72 hours. Cells used for immunostaining were exposed for 48 hours at the IC50 concentration of the pulp extract. It was determined that the pulp extract produced the highest cytotoxicity in lung and colon cancer cells, obtaining an IC50 of 352.5 and 376 μg / mL respectively. In addition, fragmentation of the nucleus appears in the cells exposed to the extracts. Statistical analysis showed that no dose-dependent behavior was observed and that the time factor of exposure to the extract was a critical factor in cytotoxicity.

Keywords : Antiproliferative effect; guava; cytochrome c; lung and colon cancer cell line..

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