Abstract

JIMENEZ-QUESADA, Karol; SCHMIDT-DURAN, Alexander; QUESADA-MONTERO, Kevin  and  MOREIRA-GONZALEZ, Ileana. Isolation of vanilla-endophytic bacteria(Vanilla planifolia) with in vitro biocontrol activity againstFusarium oxysporum f. sp. Vanillae. Tecnología en Marcha [online]. 2015, vol.28, n.2, pp.116-125. ISSN 0379-3982.

Vanillasp. genus belongs to Orchidaceae family, and V. planifolia, V. pomponaand V. tahitensis.are species of commercial interest. The quality classification of vanilla is made according to the length of the capsule and vanillin content, which is used to make food and beverage, as raw material in the pharmaceutical industry and for the production of cosmetics and perfumes, among others. Currently, root rot caused by the fungus Fusarium oxyporumf. sp. Vanillae is considered to be the biggest problem facing vanilla production, causing 30 to 52% of plant death, attacking adventitious roots and preventing this plant is able to absorb water and nutrients. The fungus cannot be eradicated by the action of chemicals that damage the viability of the plants, and because the cultivation of vanilla in agroforestry systems without the application of agrochemicals is an activity that is gaining interest among small producers country. It is for this reason why was studied the ability of control of vanilla endophytic bacteria isolated from samples from Corcovado, Puriscal, Dota and Guápiles, by testing in vitroantagonism between asylee bacteria and fungus F. oxysporum,giving results about promising candidate B1M11 to respond to pathogen attack, which was corroborated by the appearance of a halo of inhibition of fungal growth on plate.

Keywords : Agroforestry system; antagonism; biotic inductor; fermentation; organic culture system.

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