Abstract

ECHEVERRIA, Fabián; BARQUERO, Miguel  and  RODRIGUEZ, David. Root development in f1 coffe hybrids obtained by tissue culture. Agron. Costarricense [online]. 2014, vol.38, n.1, pp.67-74. ISSN 0377-9424.

F1 Hybrid seedlings, obtained by indirect somatic embryogenesis, were acclimatizated under greenhouse conditions,separated in 2 types of container: biodegradable mesh with compressed turf (Jiffy®) and the other on conventional tray with soil and rice husk. Two months later, the plantlets were transferred to bags with conventional substrate and grown under nursery conditions. Development and quality of the plants were evaluated after growing during 8 months under conventional management. It was found that 33% had straight roots, however 65% had abnormalities, such as constrictions and loops, twists, bifurcations, among others, corresponding to lesser values in length, thickness and weight, both in the radical system as well as aerial. In regard to container type, acclimatizated plants grown in Jiffy® showed better growth than those in trays; however, those on which the cover was kept during the nursery stage were less developed, as compared to those were the cover was removed. It is recommended to remove the Jiffy® cover before planting in the soil bag in the nursery, and improve the temporary immersion technique to prevent morphological abnormalities of the embryos produced.

Keywords : Aclimatization; Hybrid F1; coffee; Jiffy®; green house; in vitro; RITA®.

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