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Revista de Biología Tropical
On-line version ISSN 0034-7744Print version ISSN 0034-7744
Abstract
SEGURA-ULATE, Ismael et al. Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy. Rev. biol. trop [online]. 2022, vol.70, n.1, pp.173-189. ISSN 0034-7744. http://dx.doi.org/10.15517/rev.biol.trop..v70i1.47407.
Introduction:
Most successful cases of COVID-19 pandemic mitigation and handling have relied on extensive reverse-transcription quantitative polymerase chain reaction (RT-qPCR). However, many emerging economies have struggled with current molecular testing demands due to economic, technical and technological constraints.
Objective:
To define a potential diagnostic protocol to increase testing capacity in current and post-pandemic conditions.
Methods:
We reviewed the literature, patents and commercial applications, for alternatives.
Results:
We found a good potential in saliva samples, viral inactivation and quick RNA extraction by heating; the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity.
Conclusions:
Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations.
Keywords : emerging economies; molecular testing; SARS-CoV-2; saliva; loop mediated isothermal amplification..