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Odovtos International Journal of Dental Sciences

versão On-line ISSN 2215-3411versão impressa ISSN 1659-1046


RAMIREZ, Karol; QUESADA-YAMASAKI, Daniel  e  FORNAGUERA-TRIAS, Jaime. A Protocol to Perform Systemic Lipopolysacharide (LPS) Challenge in Rats. Odovtos [online]. 2019, vol.21, n.1, pp.53-66. ISSN 2215-3411.


Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria. In animals, intraperitoneal administration of LPS, stimulates innate immunity and the production of proinflammatory cytokines. LPS provides an inflammatory stimulus that activates the neuroimmune and neuroendocrine systems resulting in a set of responses termed sickness behavior. The purpose of this protocol is to describe step-by-step the preparation and procedure of application of intraperitoneal injection of LPS in rats, as a guide for those researchers that want to use this assay to mount an inflammatory response. LPS intraperitoneal challenge in rats has been widely used to evaluate antiinflammatory reagents and to address basic scientific questions.

Palavras-chave : Lipopolysaccharide; LPS challenge; LPS stimulation; Endotoxin; Inflammation.

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