Resumo

SALAZAR, Eduardo; HERNANDEZ, Ramses; TAPIA, Ana  e  GOMEZ-ALPIZAR, Luis. Molecular identification of the fungus Colletotrichum spp., insolated from banana (Musa spp) in high lands from Turrialba and determination of their sensitivity to postharvest fungicides. Agron. Costarricense [online]. 2012, vol.36, n.1, pp.53-68. ISSN 0377-9424.

Anthracnose is one of the most serious banana post-harvest diseases. A total of 25 Colletotrichum isolates were obtained from banana fruits grown in 5 areas in Turrialba County, Costa Rica; 5 isolates per area. Isolates were characterized on the basis of polymerase chain reaction (PCR) using species-specific primers of the ITS region and ß-tubulin, gene, and their in vitro sensitivities to the fungicides thiabendazole, imazalil and myclobutanil at 0.1, 1.0, 10.0 y 100.0 µg.ml^-1. All isolates were identified as Colletotrichum gloeosporioides and were sensitive to thiabendazole, whereas most isolates showed a marked reduction in sensitivity to imazalil and myclobutanil.

Palavras-chave : Anthracnose; PCR; postharvest; thiabendazole; imazalil; miclobutanil.

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