<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0379-3982</journal-id>
<journal-title><![CDATA[Revista Tecnología en Marcha]]></journal-title>
<abbrev-journal-title><![CDATA[Tecnología en Marcha]]></abbrev-journal-title>
<issn>0379-3982</issn>
<publisher>
<publisher-name><![CDATA[Instituto Tecnológico de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0379-39822024000100149</article-id>
<article-id pub-id-type="doi">10.18845/tm.v37i1.6661</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Efecto de una crema elaborada con plantago major en un modelo murino de herida aguda de piel]]></article-title>
<article-title xml:lang=""><![CDATA[Plantago major based ointment effect in wound healing murine model]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castro-Piedra]]></surname>
<given-names><![CDATA[Silvia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Porras-Jara]]></surname>
<given-names><![CDATA[Daniela]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Jiménez-Quesada]]></surname>
<given-names><![CDATA[Karol]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Garro-Monge]]></surname>
<given-names><![CDATA[Giovanni]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica.  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>03</month>
<year>2024</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>03</month>
<year>2024</year>
</pub-date>
<volume>37</volume>
<numero>1</numero>
<fpage>149</fpage>
<lpage>162</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0379-39822024000100149&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0379-39822024000100149&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0379-39822024000100149&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Plantago major (llantén), es una planta a la que se le han atribuido propiedades medicinales debido a la diversidad de compuestos que presenta. Está comprobado que extractos crudos de esta especie, pueden ayudar a la regeneración de la piel, estimular la proliferación celular y que tienen actividad antibacterial. Por lo anterior, en la presente investigación se buscó analizar la capacidad regenerativa de diferentes extractos de P. major en un modelo in vitro e in vivo, incluyendo los cultivos celulares de la planta como una alternativa biotecnológica para la producción de metabolitos de interés. Para ello, se establecieron cultivos callogénicos a partir de hojas de plantas in vitro, utilizando un medio de cultivo con 50% de sales y vitaminas M&amp;S, suplementado con 2,5 mg/l de TDZ, sacarosa 3% m/v y pH 7. Se probaron distintas concentraciones de diferentes muestras de P. major para evaluar cual era el efecto sobre la migración celular in vitro. Posteriormente, luego de seleccionar el tratamiento de extracto de campo como el más eficiente in vitro, se elaboró una crema a base de esta planta para analizar el efecto durante las diferentes fases de regeneración en piel en un modelo murino. En el caso del modelo de regeneración in vivo parece ser que el extracto de P. major ejerce una acción antiinflamatoria lo que favorece el incremento en la tasa de regeneración. De esta manera se concluye que la especie P. major podría tener un efecto positivo en la fase aguda del proceso de regeneración en piel.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Plantago major is a plant that has been attributed medicinal properties due to the diversity of compounds present in it. Crude extracts of this plant have been shown to aid in skin regeneration, stimulate cell proliferation, and exhibit antibacterial activity. This research aims to analyze the regenerative capacity of various extracts of P. major in an in vitro and in vivo model, including plant cell cultures, as biotechnology alternatives to produce metabolites of interest. Callus cultures were established in vitro using plant leaves cultured in M&amp;S medium containing 50% salts and vitamins, supplemented with 2.5 mg/l TDZ, 3% m/v sucrose and pH 7. Different concentrations of P. major samples were tested to evaluate the in vitro cell migration. It was found that field extract treatment was the most efficient. Then, it was used to develop an ointment for analyzing its effect during skin wound healing phases in a murine model. The results of the in vivo regeneration model suggest that the P. major extract has anti-inflammatory properties which could increase the closure rate Thus, it is concluded that P. major species may be able to a positive effect on the acute phase of the skin wound healing.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Callo friable]]></kwd>
<kwd lng="es"><![CDATA[cultivo celular]]></kwd>
<kwd lng="es"><![CDATA[formulación]]></kwd>
<kwd lng="es"><![CDATA[histología]]></kwd>
<kwd lng="es"><![CDATA[regeneración de piel]]></kwd>
<kwd lng="en"><![CDATA[Cell culture]]></kwd>
<kwd lng="en"><![CDATA[callus]]></kwd>
<kwd lng="en"><![CDATA[histology]]></kwd>
<kwd lng="en"><![CDATA[pharmaceutical formulation]]></kwd>
<kwd lng="en"><![CDATA[skin regeneration]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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