<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0379-3982</journal-id>
<journal-title><![CDATA[Revista Tecnología en Marcha]]></journal-title>
<abbrev-journal-title><![CDATA[Tecnología en Marcha]]></abbrev-journal-title>
<issn>0379-3982</issn>
<publisher>
<publisher-name><![CDATA[Instituto Tecnológico de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0379-39822015000500015</article-id>
<article-id pub-id-type="doi">10.18845/tm.v28i5.2216</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Irradiación subletal de fibroblastos murinos 3T3 con rayos X para su utilización como capa celular alimentadora]]></article-title>
<article-title xml:lang="en"><![CDATA[X-ray subletal irradiation of murine 3T3 fibroblasts for feeder-layer]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ramírez-Téllez]]></surname>
<given-names><![CDATA[Andrea]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Centeno-Cerdas]]></surname>
<given-names><![CDATA[Carolina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Conejo-Solís]]></surname>
<given-names><![CDATA[Mario A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Calvo-Castro]]></surname>
<given-names><![CDATA[Laura A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Ciencia e Ingeniería de los Materiales ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<volume>28</volume>
<fpage>15</fpage>
<lpage>26</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0379-39822015000500015&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0379-39822015000500015&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0379-39822015000500015&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Los queratinocitos, las principales células de la epidermis, requieren una capa alimentadora de células viables mitóticamente inactivadas para su supervivencia y proliferación in vitro. Para la producción de capas alimentadoras a partir de fibroblastos murinos 3T3, se evaluaron cuatro dosis de radiación con rayos X. Los efectos de las dosis se evaluaron mediante recuentos celulares y determinación de la viabilidad (MTT), adhesión (eficiencia de plateo) y migración celular (scratch wound healing). Además, se encontraron evidencias que sugieren el arresto mitótico mediante una tinción nuclear (GIEMSA). Se observó que una dosis de radiación correspondiente a 80 Gy generó daños suficientes en la línea celular para causar el arresto mitótico, manteniendo a la vez la actividad metabólica basal durante 25 días.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Keratinocytes, the main cells of the epidermis, require mitotically inactive but live feeder-layer cells for their survival and proliferation. We evaluated four X-ray irradiation doses on 3T3 murine fibroblasts for feeder-layer production. Each dose effect was evaluated by cell counting and cellular viability determination (MTT), cell adhesion (plating efficiency) and cell migration (scratch wound healing). Mitotic arrest was suggested by nuclear staining (GIEMSA). We determined that an 80 Gy X-ray dose generated enough cell damage to cause mitotic arrest yet keeping metabolically active cells up to 25 days.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Capa alimentadora]]></kwd>
<kwd lng="es"><![CDATA[rayos X]]></kwd>
<kwd lng="es"><![CDATA[queratinocitos]]></kwd>
<kwd lng="es"><![CDATA[MTT]]></kwd>
<kwd lng="es"><![CDATA[migración celular]]></kwd>
<kwd lng="en"><![CDATA[Feeder-layer]]></kwd>
<kwd lng="en"><![CDATA[X-rays]]></kwd>
<kwd lng="en"><![CDATA[keratinocytes]]></kwd>
<kwd lng="en"><![CDATA[MTT]]></kwd>
<kwd lng="en"><![CDATA[cell migration]]></kwd>
</kwd-group>
</article-meta>
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