<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0034-7744</journal-id>
<journal-title><![CDATA[Revista de Biología Tropical]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. biol. trop]]></abbrev-journal-title>
<issn>0034-7744</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0034-77442019000400708</article-id>
<article-id pub-id-type="doi">10.15517/rbt.v67i4.34793</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Cuantificación de hormonas 17&#946; - estradiol y progesterona en el caracol rosa Lobatus gigas (Mollusca, Gastropoda)]]></article-title>
<article-title xml:lang="en"><![CDATA[Quantification of 17&#946; - estradiol and progesterone hormones in the pink snail Lobatus gigas (Mollusca, Gastropoda)]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chong-Sánchez]]></surname>
<given-names><![CDATA[Fabiola]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Enríquez-Díaz]]></surname>
<given-names><![CDATA[Martha]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Aldana-Aranda]]></surname>
<given-names><![CDATA[Dalila]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro de Investigación y de Estudios Avanzados - Unidad Mérida, Laboratorio de Biología y Cultivo de Moluscos  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>09</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>09</month>
<year>2019</year>
</pub-date>
<volume>67</volume>
<numero>4</numero>
<fpage>708</fpage>
<lpage>715</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0034-77442019000400708&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0034-77442019000400708&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0034-77442019000400708&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN La cuantificación de las hormonas esteroides en moluscos se lleva a cabo con diferentes técnicas utilizando la hemolinfa o gónadas. Lobatus gigas es un gasterópodo de interés comercial en el Caribe, indexado en CITES como una especie protegida. Los estudios hormonales de esta especie aún no están disponibles. Por lo tanto, el objetivo de este estudio fue determinar la presencia de las hormonas esteroides 17ß - estradiol y progesterona en L. gigas a través de un método no invasivo y comparar dos técnicas para su cuantificación. Cada dos meses en un período de un año, se recolectaron las heces de diez organismos en Xel-Há, Quintana Roo, México. Las muestras se analizaron con cromatografía líquida de alta resolución y EIA. Los valores más altos de ambas hormonas se presentaron de marzo a septiembre y disminuyeron en noviembre y enero. La comparación de las concentraciones obtenidas con HPLC y EIA, mostró que los resultados son similares para el 17&#946;-estradiol (Passing - Bablock r = 0.673; -0.17 ng / ml). En contraste, los resultados de la progesterona con ambas técnicas no mostraron ajuste (Passing - Bablock r = 0.389; -1.43 ng / ml). Nuestros resultados sugieren que la técnica de EIA es adecuada para el estudio de hormonas en esta especie. El conocimiento generado permitirá monitorear y seleccionar organismos reproductores que se encuentren acondicionados en laboratorios y de esta manera no incidir en la recolecta de masas ovígeras silvestres.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT Quantification of steroid hormones in molluscs is performed with different techniques, using the hemolymph or gonads. Lobatus gigas is a Caribbean gastropod of commercial interest indexed in CITES as a protected species. Hormonal studies of this species are as yet unavailable. The objective of this study is to determine the presence of the steroid hormones 17ß - estradiol and progesterone in L. gigas using a non-invasive method, and to compare two techniques for their quantification. Every two months over the course of one year, the feces of ten organisms were collected in Xel-Ha park Quintana Roo, México. The samples were analyzed with High resolution liquid chromatography and Enzyme-linked immunosorbent assays. The values of both hormones were highest during the months of March to September then decreased during November and January. Comparison of the concentrations obtained with HPLC and EIA, presented similar results for 17&#946;-estradiol (Passing - Bablock r = 0.673; mean differences -0.17 ng / ml). In contrast, the progesterone results with both techniques showed no adjustment (Passing - Bablock r = 0.389; mean differences -1.43 ng / ml). Our results suggest that the enzyme-linked immunosorbent assay is suitable for the study of hormones in L. gigas. The knowledge generated will allow the monitoring and selection of breeding organisms that are conditioned in laboratories and thus will not affect the collection of wild egg masses.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[hormonas sexuales]]></kwd>
<kwd lng="es"><![CDATA[Lobatus]]></kwd>
<kwd lng="es"><![CDATA[acuacultura]]></kwd>
<kwd lng="es"><![CDATA[reproducción]]></kwd>
<kwd lng="es"><![CDATA[17ß - estradiol]]></kwd>
<kwd lng="es"><![CDATA[progesterona]]></kwd>
<kwd lng="en"><![CDATA[sexual hormones]]></kwd>
<kwd lng="en"><![CDATA[Lobatus]]></kwd>
<kwd lng="en"><![CDATA[aquaculture]]></kwd>
<kwd lng="en"><![CDATA[reproduction]]></kwd>
<kwd lng="en"><![CDATA[17ß - estradiol]]></kwd>
<kwd lng="en"><![CDATA[progesterone]]></kwd>
</kwd-group>
</article-meta>
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