<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2215-3411</journal-id>
<journal-title><![CDATA[Odovtos International Journal of Dental Sciences]]></journal-title>
<abbrev-journal-title><![CDATA[Odovtos]]></abbrev-journal-title>
<issn>2215-3411</issn>
<publisher>
<publisher-name><![CDATA[Facultad de Odontología. Universidad de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2215-34112018000200071</article-id>
<article-id pub-id-type="doi">10.15517/ijds.v0i0.32807</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Antibacterial Activity of Homeopathic Medications Lycopodium clavatum and Arsenicum album Against Periodontal Bacteria]]></article-title>
<article-title xml:lang="es"><![CDATA[Evaluación antibacteriana de los medicamentos homeopáticos sobre bacterias periodontales]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Almaguer-Flores]]></surname>
<given-names><![CDATA[Argelia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[González-Alva]]></surname>
<given-names><![CDATA[Patricia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,National Autonomous University of Mexico  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,The National Autonomous University of Mexico  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>08</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>08</month>
<year>2018</year>
</pub-date>
<volume>20</volume>
<numero>2</numero>
<fpage>71</fpage>
<lpage>79</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S2215-34112018000200071&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S2215-34112018000200071&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S2215-34112018000200071&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract There are several controversies regarding the efficacy of homeopathic substances; however, these remedies are used in many countries for the treatment of various pathological conditions. The purpose of this study was to evaluate the in vitro antibacterial activity of two homeopathic tinctures Arsenicum album (mineral extract) and Lycopodium clavatum (plant extract) on the periodontal bacteria Actinomyces israelii, Streptococcus sanguinis, Prevotella intermedia, Aggregatibacter actinomycetemcomitans and Phorphyromonas gingivalis (P. gingivalis). Materials and methods: Equal numbers of bacteria were seeded on agar plates containing enriched media with the homeopathic solutions at 1dH and 1cH dilutions. After 7 days of incubation under anaerobic conditions, colony forming units (CFUs) were counted. The antibacterial effect was calculated based on the total number of CFUs observed on non-tincture containing agar, and on the tincture containing plates. Results: No visible growth of any of the strains was observed on the plates containing Arsenicum album at any of the dilutions tested. In contrast, when Lycopodium clavatum at 1cH dilution was tested, only P. gingivalis was susceptible to this compound. Conclusions: The results suggest that the mineral extract tincture had a greater antibacterial activity than the plant extract tincture, also Lycopodium clavatum preparation could be an effective inhibitor of periodontal pathogens bacteria such as P. gingivalis.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Se necesita un mayor número de estudios in vitro e in vivo para validar estos resultados.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Homeopathy]]></kwd>
<kwd lng="en"><![CDATA[Plant extracts]]></kwd>
<kwd lng="en"><![CDATA[Antibacterial activity]]></kwd>
<kwd lng="en"><![CDATA[Periodontal bacteria]]></kwd>
<kwd lng="en"><![CDATA[Arsenicum album]]></kwd>
<kwd lng="en"><![CDATA[Lycopodium clavatum]]></kwd>
<kwd lng="es"><![CDATA[Homeopatía]]></kwd>
<kwd lng="es"><![CDATA[Extractos de plantas]]></kwd>
<kwd lng="es"><![CDATA[Actividad antibacterina]]></kwd>
<kwd lng="es"><![CDATA[Bacterias periodontales]]></kwd>
<kwd lng="es"><![CDATA[Arsenicum album]]></kwd>
<kwd lng="es"><![CDATA[Lycopodium clavatum]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Socransky]]></surname>
<given-names><![CDATA[S.S.]]></given-names>
</name>
<name>
<surname><![CDATA[Haffajee]]></surname>
<given-names><![CDATA[A.D.]]></given-names>
</name>
<name>
<surname><![CDATA[Smith]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Martin]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Haffajee]]></surname>
<given-names><![CDATA[J.A.]]></given-names>
</name>
<name>
<surname><![CDATA[Uzel]]></surname>
<given-names><![CDATA[N.G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Use of checkerboard DNA-DNA hybridization to study complex microbial ecosystems]]></article-title>
<source><![CDATA[Oral Microbiol Immunol.]]></source>
<year>2004</year>
<volume>19</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>352-62</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hajishengallis]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Periodontitis: from microbial immune subversion to systemic inflammation]]></article-title>
<source><![CDATA[Nat Rev Immunol.]]></source>
<year>2015</year>
<volume>15</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>30-44</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Mourão]]></surname>
<given-names><![CDATA[L.C.]]></given-names>
</name>
<name>
<surname><![CDATA[Moutinho]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Canabarro]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Additional benefits of homeopathy in the treatment of chronic periodontitis: a randomized clinical trial]]></article-title>
<source><![CDATA[Complement Ther Clin Pract.]]></source>
<year>2013</year>
<volume>19</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>246-50</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Socransky]]></surname>
<given-names><![CDATA[S.S.]]></given-names>
</name>
<name>
<surname><![CDATA[Haffajee]]></surname>
<given-names><![CDATA[A.D.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Periodontal microbial ecology]]></article-title>
<source><![CDATA[Periodontol 2000]]></source>
<year>2005</year>
<volume>38</volume>
<page-range>135-87</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Mathie]]></surname>
<given-names><![CDATA[R.T.]]></given-names>
</name>
<name>
<surname><![CDATA[Farrer]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Outcomes from homeopathic prescribing in dental practice: a prospective, research-targeted, pilot study]]></article-title>
<source><![CDATA[Homeopathy]]></source>
<year>2007</year>
<volume>96</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>74-81</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Khuda-Bukhsh]]></surname>
<given-names><![CDATA[A.R]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Towards understanding molecular mechanisms of action of homeopathic drugs: an overview]]></article-title>
<source><![CDATA[Mol Cell Biochem.]]></source>
<year>2003</year>
<volume>253</volume>
<numero>1-2</numero>
<issue>1-2</issue>
<page-range>339-45</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[de Araújo]]></surname>
<given-names><![CDATA[F.R.]]></given-names>
</name>
<name>
<surname><![CDATA[de Castro]]></surname>
<given-names><![CDATA[C.M.]]></given-names>
</name>
<name>
<surname><![CDATA[Severo]]></surname>
<given-names><![CDATA[M.S.]]></given-names>
</name>
<name>
<surname><![CDATA[MeF]]></surname>
<given-names><![CDATA[Diniz]]></given-names>
</name>
<name>
<surname><![CDATA[Viana]]></surname>
<given-names><![CDATA[M.T.]]></given-names>
</name>
<name>
<surname><![CDATA[Evêncio]]></surname>
<given-names><![CDATA[L.B]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Effect of Mercurius solubilis on the bacteriological response in the alveolitis process in rats]]></article-title>
<source><![CDATA[Homeopathy]]></source>
<year>2009</year>
<volume>98</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>160-4</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[CHOPRA]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[CHOPRA]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Homeopathy As Holistic Alternative Therapy in Periodontal Patients]]></article-title>
<source><![CDATA[International Journal of Contemporary Dentistry]]></source>
<year>2012</year>
<volume>3</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>1-4</page-range></nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Choudhary]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Kumar]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
<name>
<surname><![CDATA[Malhotra]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Singh]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Medicinal plants with potential antiarthritic activity]]></article-title>
<source><![CDATA[J Intercult Ethnopharmacol]]></source>
<year>2015</year>
<volume>4</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>147-79</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sandoval]]></surname>
<given-names><![CDATA[L.G]]></given-names>
</name>
</person-group>
<source><![CDATA[Farmacopea Homeopatica Mexicana]]></source>
<year>1996</year>
<edition>3th</edition>
<page-range>188-9</page-range><publisher-loc><![CDATA[New Delhi ]]></publisher-loc>
</nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hahnemann]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
</person-group>
<source><![CDATA[Organin de la medicina: el libro fundamental de la homeopatía/escrito por su fundador Samuel Hahnemann]]></source>
<year>2010</year>
<edition>10th</edition>
<page-range>93</page-range><publisher-loc><![CDATA[México, D.F. ]]></publisher-loc>
<publisher-name><![CDATA[Porrúa]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Iauk]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Lo Bue]]></surname>
<given-names><![CDATA[A.M.]]></given-names>
</name>
<name>
<surname><![CDATA[Milazzo]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
<name>
<surname><![CDATA[Rapisarda]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Blandino]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Antibacterial activity of medicinal plant extracts against periodontopathic bacteria]]></article-title>
<source><![CDATA[Phytother Res.]]></source>
<year>2003</year>
<volume>17</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>599-604</page-range></nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Groppo]]></surname>
<given-names><![CDATA[F.C.]]></given-names>
</name>
<name>
<surname><![CDATA[CeC]]></surname>
<given-names><![CDATA[Bergamaschi]]></given-names>
</name>
<name>
<surname><![CDATA[Cogo]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Franz-Montan]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Motta]]></surname>
<given-names><![CDATA[R.H.]]></given-names>
</name>
<name>
<surname><![CDATA[de Andrade]]></surname>
<given-names><![CDATA[ED]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Use of phytotherapy in dentistry]]></article-title>
<source><![CDATA[Phytother Res.]]></source>
<year>2008</year>
<volume>22</volume>
<numero>8</numero>
<issue>8</issue>
<page-range>993-8</page-range></nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bell]]></surname>
<given-names><![CDATA[I.R.]]></given-names>
</name>
<name>
<surname><![CDATA[Schwartz]]></surname>
<given-names><![CDATA[G.E]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Enhancement of adaptive biological effects by nanotechnology preparation methods in homeopathic medicines]]></article-title>
<source><![CDATA[Homeopathy]]></source>
<year>2015</year>
<volume>104</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>123-38</page-range></nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Banerjee]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Bhattacharyya]]></surname>
<given-names><![CDATA[S.S.]]></given-names>
</name>
<name>
<surname><![CDATA[Pathak]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Boujedaini]]></surname>
<given-names><![CDATA[N.]]></given-names>
</name>
<name>
<surname><![CDATA[Belon]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Khuda-Bukhsh]]></surname>
<given-names><![CDATA[A.R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Evidences of protective potentials of microdoses of ultra-high diluted arsenic trioxide in mice receiving repeated injections of arsenic trioxide]]></article-title>
<source><![CDATA[Evid Based Complement Alternat Med.]]></source>
<year>2011</year>
<volume>2011</volume>
<page-range>391752</page-range></nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bellavite]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Marzotto]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Olioso]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Moratti]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
<name>
<surname><![CDATA[Conforti]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[High-dilution effects revisited. 2. Pharmacodynamic mechanisms]]></article-title>
<source><![CDATA[Homeopathy]]></source>
<year>2014</year>
<volume>103</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>22-43</page-range></nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Chikramane]]></surname>
<given-names><![CDATA[P.S.]]></given-names>
</name>
<name>
<surname><![CDATA[Suresh]]></surname>
<given-names><![CDATA[A.K.]]></given-names>
</name>
<name>
<surname><![CDATA[Bellare]]></surname>
<given-names><![CDATA[J.R.]]></given-names>
</name>
<name>
<surname><![CDATA[Kane]]></surname>
<given-names><![CDATA[S.G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Extreme homeopathic dilutions retain starting materials: A nanoparticulate perspective]]></article-title>
<source><![CDATA[Homeopathy]]></source>
<year>2010</year>
<volume>99</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>231-42</page-range></nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Konrath]]></surname>
<given-names><![CDATA[E.L.]]></given-names>
</name>
<name>
<surname><![CDATA[Neves]]></surname>
<given-names><![CDATA[B.M.]]></given-names>
</name>
<name>
<surname><![CDATA[Lunardi]]></surname>
<given-names><![CDATA[P.S.]]></given-names>
</name>
<name>
<surname><![CDATA[CoS]]></surname>
<given-names><![CDATA[Passos]]></given-names>
</name>
<name>
<surname><![CDATA[Simões-Pires]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Ortega]]></surname>
<given-names><![CDATA[M.G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from South America on alkaloid extracts]]></article-title>
<source><![CDATA[J Ethnopharmacol.]]></source>
<year>2012</year>
<volume>139</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>58-67</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
