<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0379-3982</journal-id>
<journal-title><![CDATA[Revista Tecnología en Marcha]]></journal-title>
<abbrev-journal-title><![CDATA[Tecnología en Marcha]]></abbrev-journal-title>
<issn>0379-3982</issn>
<publisher>
<publisher-name><![CDATA[Instituto Tecnológico de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0379-39822019000200091</article-id>
<article-id pub-id-type="doi">10.18845/tm.v32i2.4352</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Comparación de tres metodologías de aislamiento, purificación y cultivo in vitro de linfocitos T murinos]]></article-title>
<article-title xml:lang="en"><![CDATA[Comparison of three methodologies of isolation, purification and in vitro culture of murine T lymphocytes]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chaves-Rodríguez]]></surname>
<given-names><![CDATA[María I.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morales-Sánchez]]></surname>
<given-names><![CDATA[Johan E.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mata-Rojas]]></surname>
<given-names><![CDATA[Nazareth]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Calvo-Castro]]></surname>
<given-names><![CDATA[Laura A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández-Carvajal]]></surname>
<given-names><![CDATA[Erick]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Loaiza-Montoya]]></surname>
<given-names><![CDATA[Randall]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Tecnológico de Costa Rica Ingeniero en Biotecnología Centro de Investigación en Bio tecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Tecnológico de Costa Rica Ingeniero en Biotecnología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Tecnológico de Costa Rica Centro de Investigación en Biotecnología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Tecnológico de Costa Rica Investigador Adjunto Doctora en Ciencias Naturales. Cen tro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af5">
<institution><![CDATA[,Tecnológico de Costa Rica Centro de Investigación en Biotec nología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af6">
<institution><![CDATA[,Centro Nacional de Innovaciones Biotecnológi ca  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<volume>32</volume>
<numero>2</numero>
<fpage>91</fpage>
<lpage>99</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0379-39822019000200091&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0379-39822019000200091&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0379-39822019000200091&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen El aislamiento y selección de linfocitos T puros de cultivo primario de bazo de ratón puede obtenerse de manera rápida y fácil utilizando kits comerciales con microesferas magnéticas para capturar y purificar las células. A pesar de estas ventajas, la carga financiera que puede representar para el presupuesto de un laboratorio, la adquisición constante de estos kits justifica la evaluación de otras opciones de menor costo. Por ello, se comparó el porcentaje de recuperación celular, viabilidad y pureza de tres métodos de aislamiento y purificación de linfocitos T; dos de bajo costo y fácil implementación (aislamiento con amortiguador de amonio-cloruro-potasio (ACK)-adhesión celular y aislamiento con Ficoll), y un kit comercial de selección negativa con microesferas magnéticas. Con el método de amortiguador ACK-adhesión celular se obtuvo un porcentaje de recuperación y purificación de linfocitos T viables comparable al obtenido con el kit. Aunque este método requiere más tiempo, dado que las células deben ser incubadas durante toda la noche, es de fácil implementación y tiene una eficiencia comparable al kit, así como un menor costo. Este método puede ser tomado en cuenta para utilizar en experimentos que puedan trabajar con linfocitos activados.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract The isolation and selection of pure T lymphocytes from spleen primary culture can be obtained easily and quickly using commercial kits that use magnetics microbeads to capture and purify the cells. Despite these advantages, the financial burden that the constant acquisition of these kits can impose to a laboratory budget makes it important to evaluate other options. We compared the cell recovery, viability and purity percentage of three isolation and purification methods; two of them of low cost and easy implementation (cell adhesion of cultures treated with Ammonium-Chloride-Potassium (ACK) buffer, and Ficoll), and a commercial with kit of negative selection with magnetics microbeads. With the ACK-cell adhesion method, we found a recovery and purity percentage of lymphocytes T comparable to the one obtained with the kit. Although this method requires more time, as cells must be incubated overnight, it is easily implemented and has a comparable efficiency to the kit, as well as a lower cost. This method can be taken into account for experiments that can use activated cells.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[CD3, amortiguador ACK]]></kwd>
<kwd lng="es"><![CDATA[adherencia celular]]></kwd>
<kwd lng="es"><![CDATA[Ficoll]]></kwd>
<kwd lng="es"><![CDATA[microesferas magnéticas]]></kwd>
<kwd lng="en"><![CDATA[CD3]]></kwd>
<kwd lng="en"><![CDATA[ACK buffer]]></kwd>
<kwd lng="en"><![CDATA[cellular adherence]]></kwd>
<kwd lng="en"><![CDATA[Ficoll]]></kwd>
<kwd lng="en"><![CDATA[magnetic microbeads]]></kwd>
</kwd-group>
</article-meta>
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