<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0379-3982</journal-id>
<journal-title><![CDATA[Revista Tecnología en Marcha]]></journal-title>
<abbrev-journal-title><![CDATA[Tecnología en Marcha]]></abbrev-journal-title>
<issn>0379-3982</issn>
<publisher>
<publisher-name><![CDATA[Instituto Tecnológico de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0379-39822018000400025</article-id>
<article-id pub-id-type="doi">10.18845/tm.v31i4.3957</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Optimización de una ruta metabólica en Escherichia coli para la producción de farneseno mediante el uso de biología sintética]]></article-title>
<article-title xml:lang="en"><![CDATA[Optimization of a metabolic pathway in Escherichia coli for the production of farnesene through the use of synthetic biology]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Garro-Monge]]></surname>
<given-names><![CDATA[Giovanni]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-Gómez]]></surname>
<given-names><![CDATA[David]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Valerín-Berrocal]]></surname>
<given-names><![CDATA[Karla]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Jiménez-Quesada]]></surname>
<given-names><![CDATA[Karol]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Centro de Investigación en Biotecnología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Centro de Investigación en Biotecnología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Centro de Investigación en Biotecnología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Centro de Investigación en Biotecnología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<volume>31</volume>
<numero>4</numero>
<fpage>25</fpage>
<lpage>36</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0379-39822018000400025&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0379-39822018000400025&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0379-39822018000400025&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen La generación de compuestos de interés utilizando residuos orgánicos como materia prima resulta en una opción económica e industrialmente viable. Mediante el uso de biología sintética es posible rediseñar microorganismos capaces de utilizar estos residuos como fuentes de carbono en la producción de biocombustibles, vacunas, alcoholes, aceites y otros. La producción de farneseno y sus precursores se hace posible mediante la modificación de microorganismos tales como Escherichia coli insertando rutas metabólicas sintéticas capaces de generar estos compuestos de interés. Luego de estudiar, optimizar y sintetizar los genes que componen la ruta metabólica del mevalonato, la cual origina el farneseno, estos fueron ensamblados junto a una serie de promotores de la familia Anderson para transformar E. coli y generar E,E,-farneseno de forma sintética.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Interest compounds generation using organic waste as raw material results in an economically and industrially viable option. Through synthetic biology is possible the design of microorganisms capable of using these residues as carbon sources in order to produce biofuels, vaccines, alcohol, oils and other products. Farnesene production and its precursors is possible through the genetic modification of microorganisms like Escherichia coli, introducing metabolic pathways capable of produce the interest compounds. After studying, optimizing and synthesizing the genes that make up the metabolic pathway of mevalonate, which originates farnesene, these were assembled together with several Anderson family promoters to transform E. coli and generate E, E, -farnesene from synthetic form.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Biología sintética]]></kwd>
<kwd lng="es"><![CDATA[farneseno]]></kwd>
<kwd lng="es"><![CDATA[RFC10]]></kwd>
<kwd lng="es"><![CDATA[ruta de mevalonato]]></kwd>
<kwd lng="es"><![CDATA[terpenoides]]></kwd>
<kwd lng="en"><![CDATA[Sinthetic biology]]></kwd>
<kwd lng="en"><![CDATA[farnesene]]></kwd>
<kwd lng="en"><![CDATA[RFC10]]></kwd>
<kwd lng="en"><![CDATA[mevalonate pathway]]></kwd>
<kwd lng="en"><![CDATA[terpenes]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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