<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0379-3982</journal-id>
<journal-title><![CDATA[Revista Tecnología en Marcha]]></journal-title>
<abbrev-journal-title><![CDATA[Tecnología en Marcha]]></abbrev-journal-title>
<issn>0379-3982</issn>
<publisher>
<publisher-name><![CDATA[Instituto Tecnológico de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0379-39822017000200097</article-id>
<article-id pub-id-type="doi">10.18845/tm.v30i2.3201</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Detección del virus del mosaico del tiquizque en aráceas comestibles mediante qPCR]]></article-title>
<article-title xml:lang="en"><![CDATA[Detection of the dasheen mosaic virus in edible aroids using qPCR]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Varela-Benavides]]></surname>
<given-names><![CDATA[Ingrid]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Montero-Carmona]]></surname>
<given-names><![CDATA[Wayner]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2017</year>
</pub-date>
<volume>30</volume>
<numero>2</numero>
<fpage>97</fpage>
<lpage>104</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0379-39822017000200097&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0379-39822017000200097&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0379-39822017000200097&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen [14] Las aráceas comestibles se consideran una fuente de calorías para personas de bajos recursos, y de ingresos para pequeños productores y microempresas, por lo que tienen gran importancia económica a nivel mundial. En Costa Rica, el tiquizque y el ñampí son importantes productos de exportación, sin embargo, al igual que en otros países, el rendimiento de las plantaciones ha disminuido y los costos de producción han crecido debido al aumento de la incidencia de patologías como el virus del mosaico del tiquizque (DsMV). La producción de plantas libres de DsMV es un importante componente en la estrategia de manejo de las aráceas comestibles, por lo que contar con un protocolo de detección rápido y confiable del virus es indispensable en tal estrategia. En este trabajo se presenta un protocolo sensible y expedito de detección del DsMV, el protocolo fue utilizado para evaluar el material de aráceas a utilizar para mejoramiento genético. Imprimadores y sonda de hidrólisis fueron diseñados basándose en las secuencias reportadas para aislamientos del virus en Nicaragua, la especificidad de los mismos fue evaluada y posteriormente se evaluó material proveniente de cultivo in vitro y material sintomático proveniente de campo. Se logró detectar con éxito la presencia del DsMV tanto en plantas sintomáticas provenientes del campo, como en plantas asintomáticas que habían sido introducidas in vitro. Este protocolo se podría utilizar para realizar más investigación en el manejo del virus y en la evaluación de materiales de exportación o importación que requieran certificación sanitaria.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract [18] Edible aroids have great economic importance worldwide since they are a source of calories for low-income people and of earnings for small farmers. The edible aroids malanga and taro are important exportation products for Costa Rica, however, as in other countries the yield of the plantations has decreased and the production costs have risen due to the increased incidence of diseases like dasheen mosaic virus (DsMV). The production of free DsMV plants is an important component of the management strategy of aroids and the fast and reliable detection of the virus is an important part of such strategy. This paper presents a sensitive and fast detection protocol of DsMV, the protocol was used to evaluate edible aroids that going to be used in a genetic improvement program. Primers and hydrolysis probe were designed based on the sequences reported from virus isolations in Nicaragua, their specificity was evaluated, and then in vitro grown plants and symptomatic material from field were evaluated. It was possible to successfully detect the presence of DsMV both in symptomatic plants from the field, as in asymptomatic in vitro plants. This protocol could be used for further research on the management of the pathology and for the evaluation of materials requiring export or import sanitary certification.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Detección del DsMV]]></kwd>
<kwd lng="es"><![CDATA[Xanthosoma]]></kwd>
<kwd lng="es"><![CDATA[qPCR]]></kwd>
<kwd lng="es"><![CDATA[aráceas comestibles]]></kwd>
<kwd lng="en"><![CDATA[DsMV detection]]></kwd>
<kwd lng="en"><![CDATA[Xanthosoma]]></kwd>
<kwd lng="en"><![CDATA[qPCR]]></kwd>
<kwd lng="en"><![CDATA[edible aroids]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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