<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0379-3982</journal-id>
<journal-title><![CDATA[Revista Tecnología en Marcha]]></journal-title>
<abbrev-journal-title><![CDATA[Tecnología en Marcha]]></abbrev-journal-title>
<issn>0379-3982</issn>
<publisher>
<publisher-name><![CDATA[Instituto Tecnológico de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0379-39822015000500069</article-id>
<article-id pub-id-type="doi">10.18845/tm.v28i5.2221</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Evaluación de técnicas de procesamiento y almacenamiento de piel cadavérica para bancos de tejidos]]></article-title>
<article-title xml:lang="en"><![CDATA[Evaluation of skin procurement and storage techniques for Tissue Banking]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Calvo-Castro]]></surname>
<given-names><![CDATA[Laura A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Guerrero-Barrantes]]></surname>
<given-names><![CDATA[Maritza]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ulloa-Fernández]]></surname>
<given-names><![CDATA[Andrea]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Portuguez-Barboza]]></surname>
<given-names><![CDATA[Rafael]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Centeno-Cerdas]]></surname>
<given-names><![CDATA[Carolina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rojas Chaves]]></surname>
<given-names><![CDATA[Miguel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Centro de Investigación en Biotecnología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Universidad de Costa Rica Departamento de Anatomía ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af5">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af6">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<volume>28</volume>
<fpage>69</fpage>
<lpage>82</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0379-39822015000500069&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0379-39822015000500069&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0379-39822015000500069&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen En el presente trabajo se reproducen y analizan algunas de las técnicas más comunes a nivel internacional para el manejo de piel cadavérica para bancos de tejidos. Para ello, se llevó a cabo la ablación, el procesamiento, la desinfección y el almacenamiento de piel utilizando un modelo animal (cerdo, Sus scrofa domesticus). Para verificar la calidad microbiológica del tejido se compararon dos diferentes métodos de desinfección (antibióticos y desinfección química con cloro). Además, se evaluó el efecto de dos tratamientos para el almacenamiento y conservación de la piel: inmersión en glicerol a bajas temperaturas (4 °C) y criopreservación (-70 °C). Finalmente, se analizó un método de esterilización utilizando irradiación con haz de electrones (25 kGy). La estructura general del tejido se analizó mediante tinciones histológicas. Los resultados demostraron la esterilización completa de las muestras, tanto de los tejidos irradiados como en la piel glicerolada desinfectada químicamente (solución de Dakins). Por otro lado, la comparación histológica de las muestras reveló que el tejido glicerolado fue el que mostró la mejor calidad en términos estructurales.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Here we report how we reproduced and analyzed the most common international techniques for managing skin for Tissue Banking. Skin ablation, processing, disinfection and storage were performed using an animal model (domestic pig, Sus scrofa domesticus). Two different disinfection methods were compared (antibiotics and chemical disinfection with chlorine) by evaluating the microbiology profile of the tissue. Also, two different storage and preservation treatments were compared: glycerol immersion at low temperatures (4°C) and cryopreservation (-70°C). Finally, we analyzed the sterilization of the tissue by electron beam irradiation (25 kGy). General structural changes in the tissue were analyzed by histological staining. Our results show that complete sterilization of the samples was achieved in both the irradiated tissue and in the chemically disinfected glycerolated skin (Dakins solution). Histological comparison between treatments revealed that samples kept in glycerol showed the best structural quality.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Irradiación con haz de electrones]]></kwd>
<kwd lng="es"><![CDATA[solución de Dakins]]></kwd>
<kwd lng="es"><![CDATA[glicerol]]></kwd>
<kwd lng="es"><![CDATA[criopreservación]]></kwd>
<kwd lng="en"><![CDATA[Electron beam irradiation]]></kwd>
<kwd lng="en"><![CDATA[Dakins solution]]></kwd>
<kwd lng="en"><![CDATA[glycerol]]></kwd>
<kwd lng="en"><![CDATA[cryopreservation]]></kwd>
</kwd-group>
</article-meta>
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