<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0379-3982</journal-id>
<journal-title><![CDATA[Revista Tecnología en Marcha]]></journal-title>
<abbrev-journal-title><![CDATA[Tecnología en Marcha]]></abbrev-journal-title>
<issn>0379-3982</issn>
<publisher>
<publisher-name><![CDATA[Instituto Tecnológico de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0379-39822015000500058</article-id>
<article-id pub-id-type="doi">10.18845/tm.v28i5.2220</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Membranas de colágeno y quitosano de fuentes alternativas: evaluación para su uso potencial en ingeniería de tejidos]]></article-title>
<article-title xml:lang="en"><![CDATA[Collagen and chitosan membranes from alternative sources: evaluation of their potential for Tissue Engineering applications]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castro-Piedra]]></surname>
<given-names><![CDATA[Silvia E.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Calvo-Castro]]></surname>
<given-names><![CDATA[Laura A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[AlvarengaVenutolo]]></surname>
<given-names><![CDATA[Silvana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Centeno-Cerdas]]></surname>
<given-names><![CDATA[Carolina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ramos-Madrigal]]></surname>
<given-names><![CDATA[Maikol]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vega-Baudrit]]></surname>
<given-names><![CDATA[José]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Zamora-Mora]]></surname>
<given-names><![CDATA[Vanessa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rojas-Chaves]]></surname>
<given-names><![CDATA[Miguel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af5">
<institution><![CDATA[,Universidad Nacional de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af6">
<institution><![CDATA[,Universidad Nacional  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af7">
<institution><![CDATA[,Universidad Nacional de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af8">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<volume>28</volume>
<fpage>58</fpage>
<lpage>68</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0379-39822015000500058&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0379-39822015000500058&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0379-39822015000500058&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Los polímeros naturales, como el colágeno y el quitosano, presentan características físicas, químicas y biológicas que los hacen buenos candidatos para usarlos como soportes análogos de matriz extracelular, con potenciales aplicaciones en la ingeniería de tejidos. En el presente trabajo se evaluaron membranas biopoliméricas de colágeno y quitosano de material considerado tradicionalmente de desecho en la industria pesquera, para el cultivo de fibroblastos dérmicos. Se emplearon matrices de 100% colágeno, 100% quitosano y colágeno:quitosano en proporciones 8:2, 2:8, 6:4 y 4:6, obtenidos a partir fuentes alternativas (cáscara de camarón y piel de tilapia). El principal reto para el empleo de estos materiales en aplicaciones biomédicas es su esterilización, para lo cual se evaluaron diferentes métodos, incluyendo tratamientos químicos (etanol, antibióticos y acetona) y físicos (rayos X, radiación UVC). El único procedimiento que permitió la desinfección efectiva sin comprometer la integridad de los biomateriales fue la incubación con antibióticos y antimicóticos durante 48 horas. Además, se confirmó la adhesión y proliferación celular sobre las membranas. Estos resultados demostraron el potencial biomédico de estos materiales.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Natural polymers such as collagen and chitosan possess physical, chemical and biological characteristics that make them good candidates as extracellular matrix scaffolds with potential applications in Tissue Engineering. In the present work, collagen and chitosan biopolymer membranes made from waste material, were evaluated for dermal fibroblasts cell culture. Several membrane compositions were analyzed, including 100% collagen, 100% chitosan, 8:2, 2:8, 6:4, 4:6 collagen-chitosan, obtained from alternative sources (shrimp shells and fish scales). Given that sterilization is one of the main challenges for using materials and devices in biomedicine, we evaluated different methods to achieve this, including chemical (ethanol, antibiotics and acetone) and physical (X-rays, UVC irradiation) treatments. Antibiotic and antifungal immersion for 48 hours was the only procedure that allowed complete sterilization without compromising the integrity of the membranes. Also, we were able to confirm cellular adhesion and proliferation on the membranes. These results demonstrate the potential of these materials to be used as scaffolds in Tissue Engineering applications.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Biopolímeros]]></kwd>
<kwd lng="es"><![CDATA[fibroblastos]]></kwd>
<kwd lng="es"><![CDATA[andamios]]></kwd>
<kwd lng="es"><![CDATA[UV]]></kwd>
<kwd lng="es"><![CDATA[rayos X.]]></kwd>
<kwd lng="en"><![CDATA[Biopolymers]]></kwd>
<kwd lng="en"><![CDATA[fibroblasts]]></kwd>
<kwd lng="en"><![CDATA[scaffolds]]></kwd>
<kwd lng="en"><![CDATA[UV]]></kwd>
<kwd lng="en"><![CDATA[X-rays]]></kwd>
</kwd-group>
</article-meta>
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<given-names><![CDATA[S. P.]]></given-names>
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<article-title xml:lang=""><![CDATA[Tissue scaffolds for skin woundhealing and dermal reconstruction.]]></article-title>
<source><![CDATA[Nanomedicine and Nanobiotechnology]]></source>
<year>2010</year>
<volume>2</volume>
<page-range>510-25</page-range></nlm-citation>
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</article>
