<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0379-3982</journal-id>
<journal-title><![CDATA[Revista Tecnología en Marcha]]></journal-title>
<abbrev-journal-title><![CDATA[Tecnología en Marcha]]></abbrev-journal-title>
<issn>0379-3982</issn>
<publisher>
<publisher-name><![CDATA[Instituto Tecnológico de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0379-39822015000500027</article-id>
<article-id pub-id-type="doi">10.18845/tm.v28i5.2217</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Cultivo in vitro de células de piel canina para su aplicación en tratamientos veterinarios]]></article-title>
<article-title xml:lang="en"><![CDATA[Culture of canine epithelial cells for veterinarian therapy]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gómez-Murillo]]></surname>
<given-names><![CDATA[Irene]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Guerrero-Barrantes]]></surname>
<given-names><![CDATA[Maritza]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Calvo-Castro]]></surname>
<given-names><![CDATA[Laura A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Centeno-Cerdas]]></surname>
<given-names><![CDATA[Carolina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rojas-Chaves]]></surname>
<given-names><![CDATA[Miguel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af5">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología Centro de Investigación en Biotecnología]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<volume>28</volume>
<fpage>27</fpage>
<lpage>32</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0379-39822015000500027&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0379-39822015000500027&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0379-39822015000500027&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen En este artículo se presenta el caso clínico de un paciente canino con un trauma a nivel de piel, que fue tratado mediante un trasplante heterólogo en una matriz gelificada de fibrinógeno con fibroblastos cultivados in vitro. La aplicación de fibroblastos en el paciente logró una mejoría notoria con respecto a la disminución del tamaño de la herida y la ausencia de reacciones alérgicas y efectos adversos frente al tratamiento. Esta experiencia abre las puertas para un desarrollo en la investigación en el área de ingeniería de tejidos veterinaria con fines terapéuticos en Costa Rica.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract We present the clinical case of a canine patient with a cutaneous trauma. The injure was treated with a heterologous transplant of in vitro cultured fibroblasts on a fibrinogen-based matrix gel. This resulted on a noticeable reduction on the wound size and the patient did not present any allergic reactions or adverse effects to the treatment. This experience opens an opportunity for the development of the veterinary Tissue Engineering area with therapeutic applications in Costa Rica.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Fibroblastos]]></kwd>
<kwd lng="es"><![CDATA[ingeniería de tejidos]]></kwd>
<kwd lng="es"><![CDATA[fibrinógeno]]></kwd>
<kwd lng="es"><![CDATA[trasplante celular]]></kwd>
<kwd lng="en"><![CDATA[Fibroblasts]]></kwd>
<kwd lng="en"><![CDATA[tissue engineering]]></kwd>
<kwd lng="en"><![CDATA[fibrinogen]]></kwd>
<kwd lng="en"><![CDATA[cellular therapy]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Cárdenas]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Garzón]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Peinado]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Modelo matemático del proceso de migración de fibroblastos en la lesión del ligamento.]]></article-title>
<source><![CDATA[Revista Cubana de Investigación Biomédica]]></source>
<year>2010</year>
<volume>29</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>1-10</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Chen]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Przyborowski]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Berthiaume]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Stem Cells for Skin Tissue Engineering and Wound Healing.]]></article-title>
<source><![CDATA[Crit Rev. Biomed. Eng]]></source>
<year>2009</year>
<volume>37</volume>
<numero>4-5</numero>
<issue>4-5</issue>
<page-range>399-421</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Freshney]]></surname>
<given-names><![CDATA[R. I.]]></given-names>
</name>
</person-group>
<source><![CDATA[Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications.]]></source>
<year>2010</year>
<edition>6</edition>
<publisher-loc><![CDATA[New Jersey, EUA ]]></publisher-loc>
<publisher-name><![CDATA[John Wiley &amp; Sons]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Groeber]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
<name>
<surname><![CDATA[Holeiter]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Hampel]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Hinderer]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Schenke-Layland]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Skin tissue engineering: In vivo and in vitro applications.]]></article-title>
<source><![CDATA[Advance Drug Delivery Reviews]]></source>
<year>2011</year>
<volume>128</volume>
<numero>2011</numero>
<issue>2011</issue>
<page-range>352-66</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Nilforoushzadeh]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Esfahani]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Fesharaki]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Siadat]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[HaftBaradaran]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Treatment of Recalcitrant Electrical Burn Ulcer with Application of Topical Trichloroacetic Acid and Autologous Cultured Fibroblast.]]></article-title>
<source><![CDATA[Cell &amp; Tissue Transplantation &amp; Therapy]]></source>
<year>2010</year>
<volume>3</volume>
<page-range>1-4</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pavletic]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[natomy and Circulation of the Canine Skin.]]></article-title>
<source><![CDATA[Microsurgery]]></source>
<year>1991</year>
<volume>12</volume>
<page-range>103-12</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ponec]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[In vitro cultured human skin cells as alternatives to animals for skin irritancy screening.]]></article-title>
<source><![CDATA[International Journal of Cosmetic Science]]></source>
<year>1992</year>
<volume>14</volume>
<page-range>245-64</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ponec]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Skin constructs for replacement of skin tissues for in vitro testing.]]></article-title>
<source><![CDATA[Advanced Drug Delivery Reviews]]></source>
<year>2002</year>
<volume>54</volume>
<numero>^sS1</numero>
<issue>^sS1</issue>
<supplement>S1</supplement>
<page-range>19-30</page-range></nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Reinke]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Sorg]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Wound Repair and Regeneration.]]></article-title>
<source><![CDATA[European Surgical Research]]></source>
<year>2012</year>
<volume>49</volume>
<page-range>35-43</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Serra]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Brazis]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Puigdemont]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Fondevila]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Romano]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
<name>
<surname><![CDATA[Torre]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Ferrer]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Development and characterization of a canine skin equivalent.]]></article-title>
<source><![CDATA[Experimental Dermatology]]></source>
<year>2007</year>
<volume>16</volume>
<page-range>135-42</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Yang]]></surname>
<given-names><![CDATA[Z.]]></given-names>
</name>
<name>
<surname><![CDATA[Xiong]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[In vitro, Tissue-Based Models as a Replacement for Animal Models in Testing of Drugs at the Preclinical Stages.]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Ceccherini-Nelli]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<source><![CDATA[Biomedical Tissue Culture]]></source>
<year>2012</year>
<publisher-name><![CDATA[InTech]]></publisher-name>
</nlm-citation>
</ref>
</ref-list>
</back>
</article>
