<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0379-3982</journal-id>
<journal-title><![CDATA[Revista Tecnología en Marcha]]></journal-title>
<abbrev-journal-title><![CDATA[Tecnología en Marcha]]></abbrev-journal-title>
<issn>0379-3982</issn>
<publisher>
<publisher-name><![CDATA[Instituto Tecnológico de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0379-39822015000500005</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Cultivo de células de piel humana sobre un soporte polimérico artificial y un soporte acelular derivado de vejiga porcina]]></article-title>
<article-title xml:lang="en"><![CDATA[Culture of human skin cells on both artificial polymeric and porcine-urinarybladder-derived acellular scaffolds]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Astúa1]]></surname>
<given-names><![CDATA[Alejandro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Lorenti2]]></surname>
<given-names><![CDATA[Alicia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Tecnológico de Costa Rica Escuela de Biología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Hospital Universitario Austral Unidad de Traducción Clínica ]]></institution>
<addr-line><![CDATA[ Buenos Aires]]></addr-line>
<country>Argentina</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>05</month>
<year>2015</year>
</pub-date>
<volume>28</volume>
<fpage>5</fpage>
<lpage>14</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0379-39822015000500005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0379-39822015000500005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0379-39822015000500005&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen La ingeniería de tejidos aplicada a lesiones de la piel propone constantemente métodos innovadores con el propósito de mejorar la calidad de vida de las personas con estas patologías. El objetivo de este estudio fue cultivar fibroblastos y queratinocitos humanos sobre soportes artificiales poliméricos termosensibles y membranas acelulares derivadas de vejiga porcina. Se establecieron cultivos primarios y subcultivos y, subsecuentemente, se obtuvieron epidermis diferenciadas y estratificadas a partir de queratinocitos que crecieron en cocultivo con una capa alimentadora (feeder layer) de fibroblastos dérmicos humanos. Mediante microscopía óptica, se efectuaron observaciones relacionadas con el crecimiento y la diferenciación de ambos tipos celulares. Se encontró que las células epiteliales cultivadas sobre soportes termosensibles produjeron láminas epidérmicas bastante semejantes a una epidermis natural, mientras que la membrana acelular derivada de vejiga porcina resultó menos exitosa para el desarrollo de queratinocitos.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Innovative methods are constantly proposed by tissue engineering in order to improve the quality of life of people affected by skin injuries. The aim of this study was the in vitro culture of human fibroblasts and keratinocytes on both artificial polymeric thermosensitive and porcineurinary-bladder-derived acellular scaffolds. Primary cultures and subcultures were established and, subsequently, differentiated and stratified epidermises were obtained from keratinocytes co-cultured with a human dermal fibroblast feeder layer. Observations on both structure and differentiation of keratinocytes and fibroblasts were performed by optical microscopy. It was observed that epithelial cells cultivated on thermosensitive scaffolds were able to produce epidermal sheets similar to normal epidermis, whereas keratinocyte development was less successful on the porcine-urinary-bladder-derived acellular membrane.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Queratinocitos]]></kwd>
<kwd lng="es"><![CDATA[fibroblastos]]></kwd>
<kwd lng="es"><![CDATA[soporte termosensible]]></kwd>
<kwd lng="es"><![CDATA[membrana acelular]]></kwd>
<kwd lng="es"><![CDATA[diferenciación]]></kwd>
<kwd lng="en"><![CDATA[Keratinocytes]]></kwd>
<kwd lng="en"><![CDATA[fibroblasts]]></kwd>
<kwd lng="en"><![CDATA[thermosensitive scaffold]]></kwd>
<kwd lng="en"><![CDATA[acellular membrane]]></kwd>
<kwd lng="en"><![CDATA[differentiation]]></kwd>
</kwd-group>
</article-meta>
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