<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0377-9424</journal-id>
<journal-title><![CDATA[Agronomía Costarricense]]></journal-title>
<abbrev-journal-title><![CDATA[Agron. Costarricense]]></abbrev-journal-title>
<issn>0377-9424</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Costa Rica. Colegio de Ingenieros y Agrónomos. Ministerio de Agricultura y Ganadería]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0377-94242021000100081</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Factores relacionados con la activación líquida de microorganismos de montaña (MM)]]></article-title>
<article-title xml:lang="en"><![CDATA[Factors related to liquid activation of mountain microorganisms (MM)]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castro-Barquero]]></surname>
<given-names><![CDATA[Leida]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[González-Acuña]]></surname>
<given-names><![CDATA[José]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Costa Rica Centro de Investi-gaciones Agronómicas-Agroalimentarias ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Departament of Plant Pathology and Microbiology-Iowa State University  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>USA</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2021</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2021</year>
</pub-date>
<volume>45</volume>
<numero>1</numero>
<fpage>81</fpage>
<lpage>92</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0377-94242021000100081&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0377-94242021000100081&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0377-94242021000100081&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Introducción. Los microorganismos de montaña (MM) son un insumo fundamental para la producción orgánica/ecológica en Costa Rica. Su uso genera preguntas relacionadas con el procedimiento para las activaciones líquidas, que presenten las mayores poblaciones microbianas. Objetivo. Evaluar factores clave que afectan la calidad de la fase líquida de MM, tales como el tiempo de activación, aireación, presencia de nutrientes, entre otros. Materiales y métodos. Se evaluaron factores asociados con las activaciones líquidas, tiempo de activación, disponibilidad de aireación, dosis de MM sólido, dosis de melaza y la adición de nutrientes inorgánicos solubles (K) e insolubles (P). Se determinaron poblaciones de bacterias, hongos filamentosos, bacterias fijadoras de nitrógeno (FN), microorganismos solubilizadores de fósforo (SP), lactobacilos (Lac) y levaduras (Lev), así como pH y P en solución. Los datos se sometieron a la prueba de Shapiro-Wilks. Las variables que no presentaron normalidad fueron evaluadas según Kruskal Wallis y las normales con ANDEVA y LSD Fisher (p&lt;0,05). Resultados y discusión. Las poblaciones cambiaron en el tiempo. El pH aumentó en el tiempo, mientras que los Lac y Lev disminuyeron. La disponibilidad de aireación redujo el tiempo de fermentación e incrementó las poblaciones, sin embargo, ocasionó un aumentó del pH en menor tiempo, por lo que los Lac y Lev disminuyeron mientras que las FN y SP incrementaron. La concentración más alta de melaza afectó negativamente la población de Lac, mientras que una reducción a la mitad en la cantidad de MM sólido, incrementó las poblaciones de SP y no afectó los otros grupos. La adición de una fuente orgánica de K aumentó las poblaciones, por tanto activados de MM reforzados pueden ser utilizados también como fuente de nutrientes. La adición de roca fosfórica no alteró las poblaciones, pero los datos sugieren que el fósforo solubilizado es retenido en la biomasa. Conclusión. Las condiciones de elaboración afectan la dinámica de las poblaciones microbianas presentes en las activaciones líquidas de MM.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Introduction. Mountain microorganisms (MM) are a fundamental input for organic/ecological farming in Costa Rica. Its use raises questions related to the procedure for liquid culture activation in order to attain the largest microbial populations. Objetive. To evaluate key factors that affect the quality of MM liquid phase culture, such as activation time, aeration, presence of nutrients, among others. Materials and methods. Factors associated with liquid-phase culture, incubation time, aeration availability, solid MM dosage, molasses dosage, and addition of soluble (K) and insoluble (P) inorganic nutrients were evaluated. Bacteria, filamentous fungi, nitrogen-fixing bacteria (FN), phosphorus-solubilizing microorganisms (SP), lactobacilli (Lac), and yeast (Lev) populations were determined, as well as pH and phosphorus in solution. Data was subjected to the Shapiro-Wilks test. Variables that did not fit a normality test were evaluated according to the Kruskall-Wallis assessment method, and when they did fit normality were analyzed by ANOVA and Fisher&#8217;s LSD test (p&lt;0.05). Results and discussion. Populations of functional microorganisms changed over time. pH increased during incubation, while Lac and Lev decreased. Increased air availability reduced fermentation time and increased microbial populations; however, this regime accelerated pH increase rate, along with faster decline of Lac and Lev, and increase of FN and SF. Higher molasses concentration negatively affected Lac populations, while halving the initial amount of solid MM resulted in increased SP populations and did not affect other microbial groups. Amending liquid culture with an organic source of K induced an increase in overall populations, therefore activated MM can also be used as a source of nutrients. Rock phosphate addition had no effect on microbial populations, but data suggests that solubilized phosphorus was fixed in microbial biomass. Conclusion. Production conditions affect the dynamics of microbial populations present in the liquid-phase activation of MM.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Microorganismos de montaña (MM)]]></kwd>
<kwd lng="es"><![CDATA[producción orgánica/ecológica]]></kwd>
<kwd lng="es"><![CDATA[bioles]]></kwd>
<kwd lng="es"><![CDATA[activaciones líquidas]]></kwd>
<kwd lng="es"><![CDATA[insumos biológicos]]></kwd>
<kwd lng="en"><![CDATA[Mountain microorganisms (MM)]]></kwd>
<kwd lng="en"><![CDATA[organic/ecological farming]]></kwd>
<kwd lng="en"><![CDATA[liquid microbial cultures]]></kwd>
<kwd lng="en"><![CDATA[liquid-phase fermentation]]></kwd>
<kwd lng="en"><![CDATA[biological inputs]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Acosta]]></surname>
<given-names><![CDATA[HA.]]></given-names>
</name>
</person-group>
<source><![CDATA[Microorganismos eficientes de montaña: evaluación de su potencial bajo manejo agroecológico de tomate en Costa Rica. Effective local soil microorganisms.]]></source>
<year>2012</year>
<page-range>100</page-range><publisher-loc><![CDATA[Turrialba, Costa Rica ]]></publisher-loc>
<publisher-name><![CDATA[CATIE]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Aguado-Santacruz]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Moreno-Gómez]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Jiménez-Francisco]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[García-Moya]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Preciado-Ortiz]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Impacto de los sideróforos microbianos y fitosideróforos en la asimilación de hierro por las plantas: una síntesis.]]></article-title>
<source><![CDATA[Rev. Fitotec. Mex.]]></source>
<year>2012</year>
<volume>35</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>9-21</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Badilla]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<source><![CDATA[Efecto de fermentos microbianos sobre el desarrollo de plántulas de caña de azucar (Saccharum officinarum L).]]></source>
<year>2019</year>
<page-range>66</page-range><publisher-loc><![CDATA[Costa Rica. ]]></publisher-loc>
<publisher-name><![CDATA[Universidad de Costa Rica,]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Castro]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<source><![CDATA[¿Cómo hacer Microorganismos de Montaña (MM)?. Receta para Productores. Brochure.]]></source>
<year>2014</year>
<page-range>6</page-range><publisher-loc><![CDATA[Costa Rica ]]></publisher-loc>
<publisher-name><![CDATA[PNAO-FITTACORI-UCR]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Castro]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Martínez]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
<name>
<surname><![CDATA[Castro]]></surname>
<given-names><![CDATA[O]]></given-names>
</name>
<name>
<surname><![CDATA[Blanco]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Abono Orgánico, Microorganismos de Montaña (MM) y Fertibiol para el control biológico de la hernia de las crucíferas (Plasmodiophora brassicae wor.) en el cultivo de mostaza china (Brassica rapa sp. pekinensis var. Taranko F1).]]></article-title>
<source><![CDATA[Agronomía Costarricense]]></source>
<year>2020</year>
<volume>44</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>31-49</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Castro]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Murillo]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Uribe]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Mata]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Inoculación al suelo con Pseudomonas fluorescens, Azospirillum oryzae, Bacillus subtilis y Microorganismos de Montaña (MM) y su efecto sobre un sistema de rotación soya-tomate bajo condiciones de invernadero.]]></article-title>
<source><![CDATA[Agronomía Costarricense]]></source>
<year>2015</year>
<volume>39</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>21-36</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Díaz-Zorita]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Fernández-Canigia]]></surname>
<given-names><![CDATA[MV]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Field performance of a liquid formulation of Azospirillum brasilense on dryland wheat productivity.]]></article-title>
<source><![CDATA[European Journal of Soil Biology]]></source>
<year>2009</year>
<volume>45</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>3-11</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Fajardo]]></surname>
<given-names><![CDATA[EE]]></given-names>
</name>
<name>
<surname><![CDATA[Sarmiento]]></surname>
<given-names><![CDATA[SC.]]></given-names>
</name>
</person-group>
<source><![CDATA[Evaluación de la melaza de caña como sustrato para la producción de Saccharomyces cerevisiae]]></source>
<year>2007</year>
<page-range>120</page-range><publisher-loc><![CDATA[Bogotá, Colombia ]]></publisher-loc>
<publisher-name><![CDATA[Pontificia Universidad Javeriana,]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hayat]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Ali]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Amara]]></surname>
<given-names><![CDATA[U]]></given-names>
</name>
<name>
<surname><![CDATA[Khalid]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Ahmed]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Soil beneficial bacteria and their role in plant growth promotion: a review.]]></article-title>
<source><![CDATA[Annals of Microbiology]]></source>
<year>2010</year>
<volume>60</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>579-98</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Khan]]></surname>
<given-names><![CDATA[AA]]></given-names>
</name>
<name>
<surname><![CDATA[Jilani]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Akhtar]]></surname>
<given-names><![CDATA[MS]]></given-names>
</name>
<name>
<surname><![CDATA[Saqlan]]></surname>
<given-names><![CDATA[SM]]></given-names>
</name>
<name>
<surname><![CDATA[Rasheed]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Phosphorus Solubilizing Bacteria: Occurrence, Mechanisms and their Role in Crop Production]]></article-title>
<source><![CDATA[Journal of Agriculture and Biological Sciences]]></source>
<year>2009</year>
<volume>1</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>48-58</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="book">
<collab>LMA-CIA</collab>
<source><![CDATA[Metodología propuesta para la evaluación de microorganismos.]]></source>
<year>2015</year>
<page-range>47</page-range><publisher-loc><![CDATA[San José, Costa Rica. ]]></publisher-loc>
<publisher-name><![CDATA[Universidad de Costa Rica,]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pacheco]]></surname>
<given-names><![CDATA[F]]></given-names>
</name>
<name>
<surname><![CDATA[Uribe]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<source><![CDATA[Lactofermento: Una alternativa en la producción de abonos orgánicos líquidos fermentados.]]></source>
<year>2006</year>
<page-range>18</page-range><publisher-loc><![CDATA[Cartago, Costa Rica ]]></publisher-loc>
<publisher-name><![CDATA[INA]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Paul]]></surname>
<given-names><![CDATA[EA.]]></given-names>
</name>
</person-group>
<source><![CDATA[Soil microbiology and biochemistry]]></source>
<year>1989</year>
<page-range>514</page-range><publisher-loc><![CDATA[Burlington, Massachusetts, USA ]]></publisher-loc>
<publisher-name><![CDATA[Elsevier]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Restrepo]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Hensel]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<source><![CDATA[El ABC de la agricultura orgánica, fosfítos y panes de piedra.]]></source>
<year>2015</year>
<page-range>397</page-range><publisher-loc><![CDATA[Cali, Colombia, ]]></publisher-loc>
<publisher-name><![CDATA[Jairo Restrepo Rivera.]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tencio]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<source><![CDATA[Guía de elaboración y aplicación de bioinsumos para una producción agrícola más sustentable.]]></source>
<year>2015</year>
<page-range>14</page-range><publisher-loc><![CDATA[San José, Costa Rica, ]]></publisher-loc>
<publisher-name><![CDATA[PNAO-MAG.]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Van Der Heijden]]></surname>
<given-names><![CDATA[MG]]></given-names>
</name>
<name>
<surname><![CDATA[Bardgett]]></surname>
<given-names><![CDATA[RD]]></given-names>
</name>
<name>
<surname><![CDATA[Van Straalen]]></surname>
<given-names><![CDATA[NM.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems.]]></article-title>
<source><![CDATA[Ecology letters]]></source>
<year>2008</year>
<volume>11</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>296-310</page-range></nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Vargas-Barrantes]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Castro-Barquero]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Aislamiento y evaluación se microorganismos solubilizadores de fósforo de Andisoles de Costa Rica.]]></article-title>
<source><![CDATA[Agronomía Costarricense]]></source>
<year>2019</year>
<volume>43</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>47-68</page-range></nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Vega]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<source><![CDATA[Curvas de absorción de nutrientes y fertilización de almácigos orgánicos de papaya (Carica papaya) híbrido Pococí, Alajuela, Costa Rica]]></source>
<year>2017</year>
<page-range>54</page-range><publisher-loc><![CDATA[San José,, Costa Rica ]]></publisher-loc>
<publisher-name><![CDATA[Universidad de Costa Rica]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Xu]]></surname>
<given-names><![CDATA[Z]]></given-names>
</name>
<name>
<surname><![CDATA[Qu]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Liu]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Duan]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Wang]]></surname>
<given-names><![CDATA[X]]></given-names>
</name>
<name>
<surname><![CDATA[Brown]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[George]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[Zhang]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Feng]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Carbon addition reduces labile soil phosphorus by increasing microbial biomass phosphorus in intensive agricultural systems.]]></article-title>
<source><![CDATA[Soil Use Management]]></source>
<year>2020</year>
<volume>36</volume>
<page-range>536-46</page-range></nlm-citation>
</ref>
<ref id="B20">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Yana]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Marschnerc]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Caoa]]></surname>
<given-names><![CDATA[W]]></given-names>
</name>
<name>
<surname><![CDATA[Zuoa]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Qina]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Influence of salinity and water content on soil microorganisms. International soil and water conservation]]></article-title>
<source><![CDATA[Research]]></source>
<year>2015</year>
<volume>3</volume>
<page-range>316-23</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
