<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0034-7744</journal-id>
<journal-title><![CDATA[Revista de Biología Tropical]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. biol. trop]]></abbrev-journal-title>
<issn>0034-7744</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Costa Rica]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0034-77442019000200078</article-id>
<article-id pub-id-type="doi">10.15517/rbt.v67i2supl.37207</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[How many larvae and nymphs of Amblyomma spp. ticks (Ixodida: Ixodidae) are needed to detect the presence of Rickettsia (Rickettsiales: Rickettsiaceae)?]]></article-title>
<article-title xml:lang="es"><![CDATA[¿Cuántas larvas y ninfas de la garrapata Amblyomma spp. (Ixodida: Ixodidae) se requieren para detectar la presencia de la bacteria Rickettsia (Rickettsiales: Rickettsiaceae)?]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Barrantes]]></surname>
<given-names><![CDATA[Gilbert]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Sánchez-Quirós]]></surname>
<given-names><![CDATA[Catalina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Costa Rica  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Costa Rica</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<volume>67</volume>
<numero>2</numero>
<fpage>78</fpage>
<lpage>82</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_arttext&amp;pid=S0034-77442019000200078&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_abstract&amp;pid=S0034-77442019000200078&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.sa.cr/scielo.php?script=sci_pdf&amp;pid=S0034-77442019000200078&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract 16. Tick-borne rickettsiosis is becoming a common emergent disease in many tropical countries, and the rapid detection of this disease could reduce the impact on wild life and public health. Pools of larvae and nymphs, and individual adult ticks are used for detection of Rickettsia species (Rickettsiaceae), but the number of larvae and nymphs in the pools likely affect the probability of detection of Rickettsia spp. We tested whether the number of larvae or nymphs of Amblyomma (Ixodidae) included in the pools affected the probability of detection. The number of larvae in each pool did not affect the probability of detecting Rickettsia spp., but the probability of detection increased rapidly with the number of nymphs. In this study, 20 nymphs are enough to approach a probability of 1 of detecting Rickettsia.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen 20. La rickettsiosis transmitida por garrapatas es una enfermedad emergente cada vez más común en muchos países tropicales, por lo que su rápida detección podría reducir su impacto negativo en la salud humana. Grupos (pozos) de larvas y ninfas de garrapatas, así como individuos adultos son usados para detectar la presencia de diferentes especies de Rickettsia. Sin embargo, el número de larvas y ninfas incluidos en estos grupos probablemente afecta la detección de Rickettsia spp. Nosotros probamos si el número de larvas o ninfas incluidas en estos grupos afecta la probabilidad de detección. Encontramos que el número de larvas incluidas en cada grupo no afectó la probabilidad de detección de Rickettsia spp., pero la probabilidad de detección aumenta rápidamente con el número de ninfas incluidas en los grupos. En este estudio, 20 ninfas por grupo fueron suficientes para tener una probabilidad cercana a 1 de detectar Rickettsia.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Amblyomma]]></kwd>
<kwd lng="en"><![CDATA[larva and nymph pools]]></kwd>
<kwd lng="en"><![CDATA[Rickettsia]]></kwd>
<kwd lng="es"><![CDATA[Amblyomma]]></kwd>
<kwd lng="es"><![CDATA[Número de larvas y ninfas]]></kwd>
<kwd lng="es"><![CDATA[Rickettsia]]></kwd>
</kwd-group>
</article-meta>
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<page-range>101-6</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
