0034-7744 0034-7744 S0034-77441998000400017 Nigeria 00 12 1998 00 12 1998 46 4 1109 1115

Effect of saline solutions and salt stress on seed germination
of some tropical forest tree species
 
 
D. A. Agboola 1
 
 
Received 4-III-1997. Corrected 21-IX-1998. Accepted 5-X-1998.
 
 
Abstract ]]> The effect of several saline solutions (as given by six salts) and salt stress (as given by 0.1 - 2m sodium chloride solutions) on the germination of seeds of six selected tropical forest tree species was investigated. Saline solutions (0.2m) of the six salts used had highly significant effects on seed germination in most of the tree species. Sodium sulphate (Na2So4) permitted germination in the seeds of Ceiba pentandra and Tectona grandis presoaked in its 0.2m solution for 36 and 48h respectively. The Zinc sulphate (ZnSO4) solution enhanced the germination of seeds of Terminalia ivorensis and Terminalia superba. Solution of Potassium per Manganate favoured the germination of seeds of T. grandis, T. ivorensis and T. superba. In general, increase in molar concentration of NaCl adversely affected the germination rate of seeds. Sees of the two Terminalia species could withstand NaCl salt stress. Seeds of T. grandis have high ability to withstand salt stress comparatively.
 

Key words

Saline solutions, salt stress, forest trees, seed germination.
 

In tropical soils, underground water which forms the major source of water supply for the forest trees is subjected to frequent salinisation. When the evaporative power of air is high and when water is being pumped out from the saline water table, the surface inch of the soil frequently becomes more saline than the subsurface (El - Sharkawi and Springuel 1979). In the tropics also, saline soils are typified by lagoons and mangrove swamps. In some wet rainforest near the sea, toxic concentrations do not often occur in such soils because the soluble constituents are carried away in drainage water to the water table below the root range (Ewusie 1980).

A soil is saline when its total soluble salt content is excessive. That is when there are enough salts to affect plant growth adversely. Growth of sensitive plants becomes impaired when the salt content of the soil exceeds about 0.1%. Various salts of which Sodium, Calcium and Magnessium are most common, contribute to Salinity. High levels of fertilization also contribute to salt accumulation and can be significant in agricultural situations (Treshow 1970). Soil salinity affects soil water potentials, pH, microbial acitvity. High concentrations of salts are expected in arid Land ecosystems, but salinity is also important in some expected situations such as heavily forested river courses in these saline regions of the tropics (Pearcy 1994).

Therefore the seed is generally in a more saline environment than the established plants whose roots can use less saline portion of the soil profile. The factor of high evaporation, transpiration and concentration of salts on soil surface coupled with greater inherent sensitivity to salt are responsible for most emergence failures on these saline soils of the coastal as well as the arid and semi arid regions of the tropics (Ayers 1952, Ewusie 1980).

The tropical tree species investigated include Ceiba pentandria, Terminalia ivorensis, Terminalia superba, Tectona grandis, Gmelina arborea and Leucaena leucocephala. The first three are indigenous timber producing trees of the rainforest belft of Nigeria, while the latter three are exotic species introduced from some other tropical zones (especially South East Asia) and thrive well in the Savannah and rainforest regions of Nigeria. Leucaena has been of immense importance in various agroforestry practices in Nigeria for Nitrogen fixation and therefore adding nutrients to the soil and crops in alley cropping (Kang 1987).

Most of the tree species especially T. superba and T. ivorensis face the problem of extinction due to over exploitation (Onochie 1991). The aim of this investigation is to study the effect of salt strees and saline conditions on the germination of seeds of six of the tropical tree species commonly in use in Nigeria and other tropical zones of the world. The information from this study will hopefully enhance various nursery practices with a view to having successful germination and seedling procurement. ]]>  

Materials and Methods

The effect of six several saline solutions (0.2m) mostly encountered in tropical soils, was first studied assuming that tropical soil solutions contain mixtures of most of these salts (Shabassy et al. 1970, Treshow 1970). This was followed by the study of the effect of different levels as given by 0.1 - 2m sodium chloride (NaCl) solutions on the germination of seeds. The choice of NaCl is based on its common occurence and stability in tropical soils, where these tree species grow (Ungar 1962, Nelson 1965).

Dry pretreated (dormancy - free) seeds in each case were surfaced sterilized with 0.1% mercuric chloride solution for 10 seconds and then rinsed several times with distilled water. Seeds were divided into lots and pre-soaked in 0.2M solutions of Sodium chloride (NaCl), Potassium permanganate (KMNO4), Ammonium Chloride (NH4Cl), Sodium Sulphate (Na2SO4), Zinc Sulphate (ZnSO4) and Calcium Carbonate (CaCO3), for period of 0, 24, 36 and 48 hours. The effect of the solutions gave osmotic pressures of between 4 to 15 x 105 pascals (Ungar 1962, El-sharkawi et al. 1979). Water served as the control.

The seeds under each treatment were then prepared for germination, for which 25 seeds were plated in 9cm (except T. grandis in 20cm) sterile glass Petri dishes lined with sterile filter papers and moistened with 20ml sterile distilled water. Five replicates from each treatment were placed in germination cabinets running at constant temperature of 30+oC and an alternating day/night regime of 30/27°C. Twelve hours periods of lights were used per day and seeds were watered when necessary.

In another experiment, different lots of the pretreated seeds of the six species were plated for germination as earlier described. However, instead of distilled water, seeds in the petri dishes were moistened with 20ml of Nacl solution. Solutions (0.1 to 2M) giving a total of 12 treatments, with water serving as the control. The solutions of NaCl were prepared according to the methods described by Devlin (1969) (Table 1). The experimentation was randomized block design. The mean values of 5 replicates were calculated and data subjected to analysis of variance (ANOVA). Moreover the significant differences between treatment means and control were compared by the least significant differences test (LSDT) (P = 0.05).
 

TABLE   1
Molar Concentrations of Sodium chloride solutions used
]]> and their corresponding osmotic pressures.

 
 

Molar Concentration of NaCl (M) 
Osmotic Pressure 
(x 105 pascals) 
Osmotic Pressures (ATM) 
     
0.0(H2O) 
 
 ]]>  
0.0
0.0
0.1 
3.84 
3.8 
0.2 
7.68 
7.6 
0.3 
 ]]> 11.51 
11.4 
0.4 
15.35 
15.2 
0.5 
15.4 
19.2 
0.6 
22.6 
 ]]> 22.4 
0.7 
26.8 
26.6 
0.8 
30.7 
30.4 
0.9 
34.5 
34.2 
]]> 1.0 
38.4 
38.0 
2.0 
72.7 
72.0 
 
 
Results

The results of the effect of six different salt solutions on the total percentage of germinated seeds of six tropical tree species are as shown on tables 2 and 3. It was observed that various solutions had a highly significant effect on the germination of seeds in most of the tree species.

Ceiba pentandra showed that 0.2M Na2SO4 solution decreased seed germination. Thus, over 43 - 52% germination was recorded in seeds of C. pentandra presoaked for 36 and 48h respectively (Tables 2 and 3) Germination was as low as 3 - 10% in C. pentandra and T. superbs in other salt solutions. None of the 0.2M solutions of CaCO3 and ZnSO4 permitted germination of seeds in most of the tree species. The exceptions were T. ivorensis and T. superba where seeds pre-soaked in 0.2M solutions of CaCO3 for 24h. gave 46 and 55% of total germination. The solution of KMNO4 was observed to enhance the germination of seeds of T. grandis, T. ivorensis and T. superba (Table 3). About 70 - 100% total germination was observed in their seeds presoaked in 0.2M of this salt solution for 36 - 48h (Tables 2 & 3). ]]>

TABLE  2
Effect of different types of salt solutions on the germination of seeds of some tropical tree species.
Data are means of five replicates
Percentage Germination
                       
 
 Ceiba pentandra 
  Leucaenaleucocephala
 Gimelina arborea
  
                   
0.2M (HRS) 
24
36
48
 ]]> 24
36
48
24
36
48 
SOLUTIONS
 
 
 
 
 ]]>  
 
 
 
 
Water(Control) 
0 
94.2+2.0 
97.3+3 
0 
91.2+1 
 ]]> 93.7+1 
46.2+3 
65.2+7 
100 
NaCl 
0 
4.0+1 
6.0+2 
0 
0 
5.0+1 
 ]]> 10.1+3 
0 
0 
KMnO4
0 
3.1+1 
7.1+1 
0 
0 
0 
0 
 ]]> 72.2+3 
100 
NH4Cl 
0 
0 
0 
0 
0 
0 
0 
25.1+1 
 ]]> 0 
Na2SO4
0 
43.2+2 
52.1+3 
0 
22.1+6 
34.6+4 
0 
0 
0 
ZnSO4 ]]> 0 
5.8+1 
9.1+3 
0 
15.0+1 
22.4+4 
0 
0 
0 
CaCO3
0 
 ]]> 5.8+1 
5.0+1 
0 
0 
0 
0 
0 
0 
 
 
]]> TABLE   3
Effect of different types of salt solutions on the germination of seeds of some tropical tree species.
Data are means of five replicates
 
 
Solutions(HRS) 
24 
36 
48 
24 
 ]]> 36 
48 
24 
36 
48 
Water(Control) 
0 
94.2+2.0 
97.3+3 
0 
91.2+1 
 ]]> 93.7+1 
46.2+3 
65.2+7 
100 
NaCl 
0 
4.0+1 
6.0+2 
0 
0 
5.0+1 
 ]]> 10.1+3 
0 
0 
KMnO4
0 
3.1+1 
7.1+1 
0 
0 
0 
0 
 ]]> 72.2+3 
100 
NH4Cl 
0 
0 
0 
0 
0 
0 
0 
25.1+1 
 ]]> 0 
Na2SO4
0 
43.2+2 
52.1+3 
0 
22.1+6 
34.6+4 
0 
0 
0 
ZnSO4 ]]> 0 
5.8+1 
9.1+3 
0 
15.0+1 
22.4+4 
0 
0 
0 
CaCO3
0 
 ]]> 5.8+1 
5.0+1 
0 
0 
0 
0 
0 
0 
 
 
The total percentage of germinated seeds in all the tree species investigated was adversely affected by increase in Molar concentration of NaCl solution when compared to water. This effect is seen in the form of poor or delayed germination (Figs. 1 - 6). The results showed that T. superba, T. ivorensis and T. grandis seeds could withstand some salt stress of NaCl though the total percentage germination was decreased in the stronger solution (0.9 - 1.0M) (figs. 3 & 4). There was no germination of seeds under 2.0M NaCl solution except those of L. leucocephala and T. grandis where 18 - 36% total germination was recorded (figs. 1 & 6). The results in general showed that seeds of T. grandis have high ability to withstand salt stress when compared with others (figs. 1 & 6). ]]>  
 


 

 


 

 


  ]]> Discussion

Salt solutions with concentration as low as 0.2M did not permit germination in seeds of the six tree species with the exception of NaSO4, KMNO4 and NaCL (Tables 2 & 3). Increase in concentration of NaCl up to 1M and 2M affected germination adversely as shown in the form of delayed germination and very low percentage germination. However, seeds of T. grandis and Terminalia species could withstand stress solutions of relatively high molar concentration of NaCl (figs. 3, 4 & 6). Some of the results obtained in this study are similar to those of some other workers who showed that in general, increased salinity results in decrease in germinability and delayed rate of germination (Palmer et al. 1969, Alekseeva 1973, Elsharkawi and Springuel 1979). Sonaike and Okusanya (1987) showed that salinity caused a loss in germination of Lufa aegyptica seeds at a concentration of 10% sea water. They found that germination decreased as salinity increased. This is also true of Eucalyptus degulpta and E. camaldulensis - (Vandermoezel and Bell 1987).

The effect of salts in solution on germination of seeds is generally complex. Discrepancies are numerous as limits between predominant osmotic effect and the toxic effect of one and same salts are not defined. Solution of Na2SO4 decreased germination especially in seeds of C. pentandra and L. leucocephala (Table 2). The promotion of germination by some salts in solution has been demonstrated many times (Devlin 1969). The most popular and widely used is Potassium nitrate. This enhancement effect may be via testa tissue destruction facilitating imbibition of water to germination (Heydecker 1980).

There are reports that the solutions treatments such as those of methylbromide have lead to low percentage germination of each of the seeds because they block the oxidative pathways leading to death of their embryo (Ungar 1962, Shabassy et al 1970, Declerk 1986, Hamson et al 1987). Salinity stress could operate by inactivating some hormone or enzyme systems and moreover membrane permeability may be affected in the seeds leading to a loss of germinability (Khan 1980). Soils at certain water potentials have a more drastic effect on the rate and percentage of germination (Heydecker 1980). Sodium and potassium have been found to be inhibitory and moreover inhibition of germination by iso-osmotic potentials of different substrates varied widely (Johnston 1977).

Excessive concentrations particularly of salts as calcium and sodium can alter the O.P of the soil solution to a degree which is toxic to seeds and plants and can also cause growth and supression - (Treshow 1970). The inhibition effect of salinity on seed germination may be via its inhibitory effect on seed - protein reserve mobilisation due more to the inhibition of the translocation of hydrolysis products - as it is the case in Vignia Sinensis seeds (Prisco & Vieira 1976).
 

Acknowledgements

The author is grateful to the technical staff of the Seed Store and Library of the Forestry Research Institute of Nigeria (FRIN) Ibadan for their assistance during the course of study.
 

References ]]>

Agboola, D.A. & E.O. Etejere 1991. Studies on seed dormancy of selected economic tropical forest tree species. Nigerian. J. Bot. 4: 115 - 126.         [ Links ]

Ayers, A.D. 1952. Seed germination as affected by soil moisture and Salinity. Agron. J. 44: 82 - 84.         [ Links ]

Devlin, M. 1969. Physiology. Van Nostrand Reinhold, New York. p. 41 - 43.         [ Links ]

El-sharkawi, H.M. & I.V. Springuel 1979. Germination of some crop plant seeds under salinity stress. Seed Sci. Technolo. 15: 151 - 162.         [ Links ]

Ewusie, J.Y. 1980. Elements of tropical ecology. Heineman, New Hampshire, Connecticutt.p. 134 - 135.         [ Links ]

Hamson, P.R., H.E. Vamman & C. Chrabati 1987. The effect of Methyl bromide on some varieties of barley. Seed Sci. Technol. 15: 151 - 162.         [ Links ]

Heydecker, W. 1980. Stress and seed germination an agronomic view, p. 237 — 276. In A.A. Khan (ed.). Physiology and Biochemistry of seed dormancy and germination. North Holland, New York.         [ Links ]

Johnston, M.E.H. 1971. Effect of salinity and water potential on germination, p.17 - 18. In P. Thomson (ed.). Advances in research and technology of seeds. C.T.A. Wagenigen, Nederlands.         [ Links ]

Kang, B.T. & G.F. Wilson 1987. The development of alley cropping as a promising Agro - Forestry technology, p. 227 - 243. In H.A. Stepler & P.F.R. Nair (eds.). Agroforestry, a decade of development. ICRAF, Nairobi Kenya         [ Links ]

Khan, A.A. ed. The Physiology and Biochemistry of Seed dormancy and germination. North Holland, New York. P. 29 - 45.         [ Links ]

Nielson, MS. 1965. Flowering plants of West Africa. University of London, London. p. 39 - 49.         [ Links ]

Onochie, C.F.A. 1990. The dying Forest, Declining forest resources of Nigeria. Keynote address 4th annual conferences of the Botanical Society of Nigeria. University of Nigeria, Nsukka, Nigeria.         [ Links ]

Palmer, J., D.L. Beeker & S.R. Charpman 1969. Salinity tolerance studies in Rusian wild rye, Elysmus juceus. Proc. Montreal Accad. Sci. 28. 20 - 27.         [ Links ]

Parker, J.J. & H.M. Taylor. 1965. Soil strength and seedling emergence relations. Agron. J. 47: 289 - 291.         [ Links ]

Pearcy, R.W. 1994. Plant Physiological Ecology Chapman & Hall, London. P. 90.         [ Links ]

Prisco, T.T. & G.H.F. Vieira. 1976. Effect of Nacl Salinity on nitrogenous compounds and proteases during germination of Viginia sinenssis seeds. Physiol. Plant 36: 317 - 320.         [ Links ]

Shabassy, A.T., A.I. Mitkees, A.T.A. Moustafa & A.M. Mashadi. 1970. The tolerance of corn to saline conditions - 1: The effect of CaCl2. Agric. Res. Rev. Cairo 48. 58 - 72.         [ Links ]

Sonaike, A. A. & O.T. Okusanya 1987. Germination behaviour of Lufa aegyptica seeds from 19 localities in Nigeria. Seed Sci. Technol. 15. 741 - 750.         [ Links ]

Treshow. M. 1970. Mineral Toxicity. In: Environment and Plant response. McGraw-Hill, New York. P. 222 - 236.

Udovenco, G.V. & L.I. AledSeeva 1973. Effect of salinization of initial phases of plant growth physiology. Lemngrad. p. 277 - 286.         [ Links ]

Ungar, I.A. 1962. Influence of Salinity on seed germination in succulent halophytes. Ecol. J. 43. 763 - 764.         [ Links ]

Vandermoezel, R.D. & D.J. Bell 1987. The effect of Salinity on the germination of some Western Australian Eucalyptus & Melacuela species. Seed Sci. Technol. 15. 239 - 246.         [ Links ]

1 Department of Biological Sciences, University of Agriculture, Abeokuta, Ogun State, Nigeria. Fax 23439243045. ]]> 1991 4 115 - 126 1952 44 82 - 84 1969 41 - 43 1979 15 151 - 162 1980 1987 15 151 - 162. 1980 237 - 276 1971 17 - 18 1987 227 - 243 29 - 45 1965 39 - 49 1990 . 1969 28 20 - 27 47 289 - 291 1994 1976 36 317 - 320 1970 48 58 - 72 1987 15 741 - 750. 1970 222 - 236 1973 277 - 286 1962 43 763 - 764 1987 15 239 - 246