SciELO - Scientific Electronic Library Online

 
vol.37 número1Diseño e implementación de una experiencia formativa para futuros profesores de matemáticas sobre variación linealEstudio tomográfico e histológico comparativo de dos biomateriales para preservación del reborde alveolar índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Compartir


Uniciencia

versión On-line ISSN 2215-3470versión impresa ISSN 1011-0275

Uniciencia vol.37 no.1 Heredia ene./dic. 2023

http://dx.doi.org/10.15359/ru.37-1.17 

Artículo

Insecticidal activity of ethanolic plant extracts on Aedes aegypti larvae

Actividad larvicida de extractos etanólicos de plantas contra Aedes aegypti

Atividade inseticida de extratos etanólicos de plantas sobre larvas de Aedes aegypti

Víctor Álvarez-Valverde1 
http://orcid.org/0000-0001-6007-9150

Gerardo Rodríguez2 
http://orcid.org/0000-0002-1224-0277

Silvia Argüello-Vargas3 
http://orcid.org/0000-0002-3832-1323

1 Escuela de Química, Laboratorio de Fitoquímica, Universidad Nacional, Heredia, Costa Rica. Escuela de Medicina Veterinaria, Posgrado en Ciencias Veterinarias Tropicales, Maestría en Enfermedades Tropicales, Universidad Nacional, Heredia, Costa Rica. victor.alvarez.valverde@una.ac.cr, https://orcid.org/0000-0001-6007-9150* Corresponding autor

2 Escuela de Química, Laboratorio de Fitoquímica, Universidad Nacional, Heredia, Costa Rica. garodriguezr57@gmail.com, https://orcid.org/0000-0002-1224-0277

3 Escuela de Medicina Veterinaria, Posgrado en Ciencias Veterinarias Tropicales, Maestría en Enfermedades Tropicales, Universidad Nacional, Heredia, Costa Rica. silvia.arguello.vargas@una.ac.cr, https://orcid.org/0000-0002-3832-1323

Abstract:

(Objective):

The objective of this study was to determine the larvicidal activity against A. aegypti of ethanolic extracts of plants commonly found in Costa Rica.

(Methodology):

Larvicidal activity of ethanolic extracts of plants was determined in this study using the WHO/VBC/81.807: WHO/VBC/81. from 1981. An analysis PROBID for dose response was conducted to determine LC50 of the most prominent extracts.

(Results):

A total of six extracts with the highest toxicity were chosen, with the stem extract of Ipomoea cairica having the lowest LC50 of 0.0341 mg/mL, followed by I. cairica leaf extract with an LC50 of 0.121 mg/mL. The larvicidal activity of ethanolic extracts of plants was determined in this study, with an emphasis on the larvicidal potential of I. cairica as an environmentally friendly alternative for the control of A. aegypti.

(Conclusions):

The larvicidal activity for ethanolic extracts of different plants, with an emphasis on the larvicidal potential of I. cairica as an environmentally friendly alternative for the control of A. aegypti.

Keywords: Aedes aegypti; ethanolic extracts; secondary metabolites; Ipomoea cairica; larvicidal activity

Resumen:

(Objetivo):

El objetivo de este estudio fue determinar la actividad larvicida contra A. aegypti de extractos etanólicos de plantas comúnmente encontradas en Costa Rica.

(Metodología):

La actividad larvicida de extractos etanólicos de plantas fue determinada mediante la metodología WHO/VBC/81.807: WHO/ VBC/81. de 1981 de la Organización Mundial de la salud. Un análisis PROBID para determinar la respuesta a distintas dosis fue llevado a cabo y así determinar los valores de dosis letal media (LC50) para los extractos más prominentes

(Resultados):

Un total de seis extractos con la mayor toxicidad fueron escogidos, siendo el extracto de los tallos de Ipomoea cairica lo que mostraron el menor valor de LC50 con 0.0341 mg/ mL, seguido por el extracto de las hojas de I. cairica con un LC50 de 0.121 mg/mL.

(Conclusiones):

La actividad larvicida de los extractos etanólicos de plantas fue determinada en este estudio con un énfasis en el potencial de la I. cairica como una alternativa ambientalmente amigable para el control de A. aegypti.

Palabras claves: Aedes aegypti; compuestos no volátiles; I. cairica; actividad larvicida.

Resumo:

(Objetivo):

Este estudo teve como objetivo determinar a atividade larvicida contra A. aegypti de extratos etanólicos de plantas comumente encontradas na Costa Rica.

(Metodologia):

A atividade larvicida de extratos etanólicos de plantas foi determinada usando a metodologia da OMS/VBC/81.807: WHO/VBC/81. 1981 da Organização Mundial da Saúde. Uma análise PROBID para determinar a resposta a diferentes doses foi realizada para estabelecer os valores da dose letal média (LC50) para os extratos mais proeminentes.

(Resultados):

Foi escolhido um total de seis extratos com a maior toxicidade, com o extrato de hastes de Ipomoea cairica mostrando o menor valor LC50 a 0,0341 mg/mL, seguido pelo extrato de folhas de I. cairica com um LC50 de 0,121 mg/mL.

(Conclusões):

A atividade larvicida dos extratos etanólicos de plantas foi determinada neste estudo com ênfase no potencial da I. cairica como uma alternativa ambientalmente amigável para o controle de A. aegypti.

Palavras-chave: Aedes aegypti; compostos não voláteis; I. cairica; atividade larvicida.

Introduction

Aedes aegypti is one of the most important vectors in the maintenance and transmission of viruses such as chikungunya, Zika, yellow fever, Mayaro and dengue (Paniz-Mondolfi, Rodriguez-Morales, Blohm, Marquez, & Villamil-Gomez, 2016), the latter producing the most prevalent and dangerous emerging arboviral disease (Patterson, Sammon, & Garg, 2016). The Pan American Health Organization (Pan American Health Organization, 2019) has reported an increase in dengue cases in the Americas over the last four decades, and nearly 500 million people are at risk of contracting the disease. More than 1.6 million cases were recorded in the Americas in 2020 alone, of which 9,438 were reported in Costa Rica as of October of that year (Gonzalez, 2021; Pan American Health Organization, 2020).

This increase in the number of cases impacts public health and presents a high economic cost for countries because productivity and household income are reduced by disability or death, investment in vector control equipment (human resources and infrastructure) and the follow-up and treatment of sick people (World Health Organization, 2017).

Vector control is a preventive and effective way of reducing the transmission of diseases by arthropods and promoting human and economic development (World Health Organization, 2004, 2017). The main control method implemented in the Americas has been the use of pesticides based on different chemical groups, such as organochlorines, organophosphates, carbamates and pyrethroids. However, excessive pesticide use, the absence of surveillance of doses used and the susceptibility of the vector to insecticides have resulted in the emergence of resistant A. aegypti populations (Lounibos & Kramer, 2016; Manjarres-Suarez & Olivero-Verbel, 2013). The indiscriminate use of these chemicals also has harmful effects on ecosystems and public health (World Health Organization, 2017). Costa Rica does not have an insecticide susceptibility surveillance program. However, the results of sporadic studies have shown no resistance in A. aegypti to temephos or deltamethrin but resistance to cypermethrin in a strain in Orotina (Bisset et al., 2013; Calderón & Troyo, 2016; Calderón-Arguedas & Troyo, 2014; Vargas Miranda, Troyo, & Calderón Arguedas, 2019).

Considering the current situation regarding vector control, it is important to develop natural chemical alternatives that help reduce the vector population, while generating minimal resistance, and less toxic to mammals, less harmful to the environment and more affordable (Muñoz V., Staschenko, & Ocampo D., 2014; Silvério, Espíndola, Lopes, & Vieira, 2020).

Research studies based on natural active principles in the Americas have mainly focused on the larvicidal activity and repellency of essential oils (Pilon et al., 2022). However, such information is not available for Costa Rica (Hasan, Jaal, Ranson, & McCall, 2015; Leyva-Silva et al., 2017; Manjarres-Suarez & Olivero-Verbel, 2013; Muñoz V. et al., 2014; Rey & Lounibos, 2015; Vargas Miranda et al., 2019).

Therefore, the aim of the present study is to determine the larvicidal activity against A. aegypti of ethanolic extracts of plants commonly found in Costa Rica.

Methodology

This study was carried out at the Phytochemistry Laboratory in the School of Chemistry of the National University of Costa Rica.

Plant samples

The 13 selected plants fulfilled at least two of the following inclusion criteria: being a diverse source of secondary metabolites; having a known ethnomedical use for the biological control of bacteria and/or arthropods; and being widely distributed and/ or affordable in Costa Rica (Table 1).

Samples of stems, leaves and, in some cases, seeds, were collected at the Quinta Diamante Sociedad de Responsabilidad Limitada Experimental Station in Pérez Zeledón, San José. The Ipomoea cairica sample was collected in the San Miguel de Santo Domingo sector of Heredia. All the samples were collected and processed under CONAGEBIO permit no. R-012-2020-OT-CONAGEBIO. The samples were dried in an oven at 40 °C and ground in a knife mill with a 1-mm sieve.

Table 1 Plant species used by family and habit 

Species Family Habit
Ipomoea cairica Convolvulaceae Vine
Piper umbellatum Piperaceae Herb
Piper marginatum Piperaceae Shrub
Zanthoxylum setulosum Rutaceae Tree
Argemone mexicana Papaveraceae Erect herb
Bocconia frutescens Papaveraceae Shrub to small tree
Quassia amara Simaroubaceae Shrub to small tree
Neurolaena lobata Asteraceae Epiphyte
Solanum mammosum Solanaceae Shrub
Erythroxylum macrophyllum Erythroxylaceae Shrub
Croton draco Euphorbiaceae Herb or Shrub
Pimenta dioica Myrtaceae Tree
Rosmarinus officinalis Lamiaceae Shrub
Kalanchoe sp. Crassulaceae Herb-shrub

Note: derived from research.

Extraction

Three extractions of dry and ground material were performed using 95% ethanol in an ultrasonic bath at 40 °C. The extract was filtered using gravity filter paper. The collected filtrate was concentrated using a vacuum rotary evaporator at a temperature not higher than 40 °C. The extracts were then freeze dried to produce dry and stable samples. The dry samples were weighed, and homogeneous solutions of known concentrations of each extract were prepared and applied to the larvae, as described in the following section.

Evaluation of larvicidal activity

The larvicidal activity was determined using a method described in the WHO/VBC/81.807 report of the World Health Organization (World Health Organization, 1981). The third to fourth instar larvae of the Rockefeller strain of A. aegypti susceptible to insecticides were used. To ensure physiological homogeneity for each trial, the larvae were obtained from eggs that were hatched simultaneously and raised under the same feeding and environmental conditions. Four replicates per sample concentration were used, with approximately 20 larvae each and a total volume of 25 mL of the mixture methanol: water 2:98 v:v. A positive control containing 50 ppm (mg/L) temephos and a negative control containing a mixture of methanol : chlorinated tap water (2:98) were used. Mortality was read at 24 hours. The extracts were initially evaluated at a concentration of 100 ppm. As this study was exploratory, the six extracts with the highest mortality were analyzed. For plant extracts in which a mortality percentage ≥7.9% was obtained, a response evaluation was performed at concentrations ranging from 50 ppm to 400 ppm. The LC50 and LC90 were obtained by a PROBIT analysis using XLSTAT version 2021.2 software19.

Analysis and results

The evaluation results for 100-ppm extracts of the 13 plant species are shown in Table 2. Ten of the species showed a nonzero percentage of larvicidal activity at 24 hours. Ipomoea cairica, Zanthoxylum setulosum, Piper umbellatum, Rosmarinus officinalis and Kalanchoe sp. exhibited a mortality greater than or equal to 7.9%. I. cairica had the highest mortality at 100%.

In all the tests, both the positive control containing temephos and the negative control containing water (or the corresponding mixture of solvents) exhibited the expected mortality rates of approximately 100% and 0%, respectively, indicating optimal conditions for all the tests.

Figure 1 is a regression plot of the A. aegypti larval mortality versus the logarithm (base 10) of the natural insecticide concentration. The slope of the regression plot indicates the degree of homogeneity of the population response to the stimulus, that is, the higher the slope is, the higher the homogeneity of the population response to the stimulus (Leyva et al., 2008). The highest slope was obtained for I. cairica at 2.7727, followed by Z. setulosum at 2.5495. The slope obtained from the regression equation can be used to determine the concentration range over which a substance with larvicidal activity can exert a well-defined mortality effect. The slopes determined in this study were lower in comparison with literature values (Amariles Barrera, García Pajón, & Parra Henao, 2013; Leyva et al., 2008).

Table 2 Larvicidal effect produced by ethanolic extracts of plants at 100 ppm on A. aegypti Rockefeller strain larvae 

Species Plant part* Dead larvae Total larvae Mortality percentage at 24 h
Ipomoea cairica L 32 80 40.0 ± 0.0
  S 80 80 100.0 ± 0.0
Piper umbellatum L 7.0 76.0 9.2 ± 2.6
  S 8.0 81.0 9.9 ± 0.2
Zanthoxylum setulosum L 8.0 81.0 9.8 ± 3.8
  S 2.0 80.0 2.5 ± 5.0
Argemone mexicana SD 0.0 76.0 0.0 ± 0.0
  L 4.0 76.0 5.3 ± 0.1
  S 2.0 76.0 2.6 ± 3.0
Bocconia frutescens M 1.0 76.0 1.3 ± 2.6
  L 0.0 76.0 0.0 ± 0.0
  S 0.0 76.0 0.0 ± 0.0
Quassia amara L 0.0 76.0 0.0 ± 0.0
  S 0.0 76.0 0.0 ± 0.0
Neurolaena lobata L 0.0 76.0 0.0 ± 0.0
  S 2.0 76.0 2.6 ± 3.0
Solanum mammosum L 4.0 76.0 5.3 ± 0.1
  S 0.0 76.0 0.0 ± 0.0
Erythroxylum macrophyllum L 1.0 76.0 1.3 ± 2.6
  S 0.0 76.0 0.0 ± 0.0
Croton draco L 0.0 76.0 0.0 ± 0.0
  S 0.0 76.0 0.0 ± 0.0
Pimenta dioica L 0.0 76.0 0.0 ± 0.0
  S 0.0 76.0 0.0 ± 0.0
Rosmarinus officinalis L 6.0 76.0 7.9 ± 3.0
Kalanchoe sp. L 2.0 76.0 2.6 ± 3.0
+ Control (temephos)   80.0 80.0 100.0 ± 0.0
- Control (water)   1.0 80.0 1.3 ± 0.5

*Legend: L = leaves; S = stems; M = midrib; SD = seeds. The uncertainty in the mortality data are expressed as the standard deviation for four replicates. Note: derived from research.

Note: derived from research

Figure 1 PROBIT plots of the logarithmic (base 10) concentration (ppm) of the ethanolic extracts of the evaluated plants versus mortality (PROBIT units). 

The LC50 and LC90 values for the evaluated ethanolic extracts are shown in Table 3. The species I. cairica species exhibited the lowest LC50, indicating that this extract elicited the highest mortality response at low concentrations.

The results of the larvicidal study revealed a diverse mortality response in the third and fourth instar larvae of the A. aegypti Rockefeller strain to 100 mg/mL (ppm) ethanolic extracts of the selected plants. The mortality rates observed for the ethanolic extracts of I. cairica were 40.0% for leaves and 100% for stems. By contrast, the mortality rates observed for the ethanolic extracts of P. dioica, C. draco and Q. amara were 0.0% for both leaves and stems at the same concentration in all cases. This result was expected because differences in the chemical compositions of the plants affect the presence of substances with larvicidal effects in the extracts, which generates variations in larval mortality (Jirovetz et al., 2007; Mora, Castro, Chavarría, Murillo, & Poveda, 2011; Patel & Patel, 2018; Setzer et al., 2007). The ethanolic extracts with the highest larvicidal activity were those of I. cairica, followed by P. umbellatum, R. officinalis and Kalanchoe sp. Other ethanolic extracts produced mortality for only one part of the studied plant: for example, a mortality of 5.3 ± 0.1% was observed for the ethanolic extract of the leaves of S. mammosum, whereas no mortality was observed for at the same concentration of the ethanolic extract of the stems. This result suggests that secondary metabolite compositions differ among plant parts, in terms of both concentration and type (Araya, Carvajal, Alvarez, Orozco, & Rodriguez, 2017; Leal et al., 2020; Rangiah & Gowda, 2019). It is notable that, despite the extract method used that does not concentrate volatile fraction of the compounds present in the plant, it was possible to find a highly lethal components int the extract with LC50 values like the ones reported in studies where evaluate the toxicity of volatile fractions of plants such as essential oils (Pilon et al., 2022).

A significant degree of toxicity was previously reported for Z. setulosum, mainly against Artemia salina, with an LC50 of 3.9 mg/mL(Boehme, Noletto, Haber, & Setzer, 2008). Hydro distillation was used to extract terpenes, terpenoids and derivatives in the respective study, because the aim was to

Table 3 LC50 and LC90 data expressed as parts per thousand (mg/mL) for the ethanolic extract evaluated against A. aegypti larvae 

Plant LC 50 in mg/mL (*95% CI) LC90 in mg/mL (*95% CI) Diagnostic dose in mg/mL (*95% CI)
Ipomoea cairica 0.0341 0.0578 0.978
(stems) (0.0293-0.0393) (0.0496-0.0674) (0.840-1.140)
Ipomoea cairica 0.121 0.337 3.707
(leaves) (0.089-0.166) (0.246-0.462) (2.52-4.89)
Zanthoxylum setulosum 0.268 0.854 24.21
(leaves) (0.185-0.386) (0.592-1.232) (16.78-34.93)
Rosmarinus officinalis (leaves) 3.41 36.03 2709.79
  (1.12-10.36) (11.85-109.54) (891.29-8237.12)
Kalanchoe sp. 11.14 320.26 54445
(leaves) (2.405-51-605) (69.15-1483.2) (11755-252153)
Piper umbellatum (stems) 39.60 3885.69 1797603
  (6.86-228.52) (673.30-22424.70) (311483-10374157)

*CI: Confidence interval. Note: derived from research.

extract mainly essential oils, and therefore, the volatile fraction of the plant. The aim of the present study was to obtain mainly the nonvolatile fraction of plants because multiple bioactivities have been reported (Araya et al., 2017; Gutierrez, Arenas, Barrera, & Martínez, 2007; Parra et al., 2018) for a large number of secondary metabolites that are not necessarily volatile, such as polyphenols, nitrogenous compounds and alkaloids (Pilon et al., 2022). Thus, there was an important methodological difference between the two studies. In addition, toxicity was measured in the two studies using different species, both belonging to the phylum Arthropoda, but to different orders.

The significant LC50 of the ethanolic extract of Z. setulosum was lower than that of the other evaluated species; however, the ethanolic extract of I. cairica exhibited an even lower LC50. I. cairica had a relatively high diagnostic dose (DD) of 0.978 mg/mL compared to those reported for commercial insecticides, such as temephos, which can reach 1 mg/L (World Health Organization, 2009). However, as a crude extract of I. cairica was used in this study, either the compounds producing larvicidal activity were present in small proportions in the extract or a combination of various metabolites generated synergistic activity (Keziah, Nukenine, Yingyang Danga, & Esimon, 2016; Liu et al., 2002).

The slope of the PROBIT plot is higher for the ethanolic extract of P. umbellatum leaves than for the extracts of R. officinalis and Kalanchoe sp. However, the low linearity of the fit (R2 = 0.4747) reflects a weak correlation in the P. umbellatum data, that is, a poor fit between the concentration and mortality compared to that for the R. officinalis and Kalanchoe sp. extracts. The slope of the PROBIT plot is an important consideration because a large slope shows greater effects, i.e., higher mortality, at low concentrations, as shown in Figure 1.

The ethanolic extract of I. cairica was expected to exhibit lower LC50 and LC90 values than the other evaluated ethanolic extracts because the largest slope of the PROBIT curves was obtained for I. cairica. Thus, I. cairica produced similar mortalities at low extract concentrations, and the aforementioned factors made the highest contribution to the linear regression analysis. Alkaloids, carbohydrates, tannins, phenolic compounds, proteins, amino acids, terpenoids, sterols, saponins, lignans and coumarins have been found in I. cairica 34,35. The two simple coumarins that have been found in I. cairica are scopoletin (7-hydroxy-6-methoxychromen-2-one) and umbelliferone 7-(hydroxychromen-2-one) (Olga & Braz-Filho, 1997). This result is important because the toxic activity of natural extracts has been correlated with the presence of coumarins in many studies and may explain the activity reported in the present study (Ahbirami, Zuharah, Thiagaletchumi, Subramaniam, & Sundarasekar, 2014; Prabha & Nagarajan, 2016; Wang, Kim, Wang, Shu, & Ahn, 2012).

Conclusions

The plants that produced ethanolic extracts with the highest larvicidal responses (I. cairica, P. umbellatum, Z. setulosum, R. officinalis and Kalanchoe sp.) were evaluated in the last stage of the study. With the exception of R. officinalis, these plants are not used for culinary or other commercial purposes and therefore have no significant commercial value.

Natural extracts with larvicidal activity are an alternative to synthetic products in commercial use. Synthetic insecticides that do not degrade easily exert pressure on biological systems, whereas a product of natural origin is incorporated back into an ecosystem after completing its function (Greenop, Cook, Wilby, Pywell, & Woodcock, 2020).

The use of field samples, in addition to the Rockefeller strain, for testing has epidemiological utility for assessing cross-resistance to the active principles of I. cairica in Costa Rica (Haziqah-Rashid et al., 2019). The results of this study are an important contribution in the exploitation of natural extracts with larvicidal activity. As no studies have been performed to evaluate the larvicidal activity of nonvolatile plant extracts, the main approach used in this study was to evaluate essential oils instead of compounds that cannot be extracted by conventional hydro distillation methods and for which bioactivity has been reported in various cases(Boehme et al., 2008; Muñoz V. et al., 2014; Pinto, Menezes, Melo, & Feitosa, 2016). The results of this study show larvicidal activity for ethanolic extracts of different plants, with an emphasis on the larvicidal potential of I. cairica as an environmentally friendly alternative for the control of A. aegypti.

Funding

The translation of this study was funded by the ''Fund to support the dissemination of knowledge generated at National University (Fondo para apoyo a la divulgación del conocimiento generado en la UNA)''according to resolution UNA-VI-RESO-116-2021.

Universidad Nacional, Costa Rica.

Acknowledgements

Special mention to Dr. Marco Herrero Acosta who was consulted during the execution of this project, M.Sc. Allan González Herrera, who facilitated working space during part of the project. Additionally, extend to the Universidad Nacional program SIA code 0568-14 name: Laboratorio de Fitoquímica an special mention in the collaboration of this project.

Conflict of Interest

The authors declare no competing interests.

Author contribution statement

All the authors declare that the final version of this paper was read and approved.

The total contribution percentage for the conceptualization, preparation, and correction of this paper was as follows: V.A.V. 60 %., G.R.R. 20 % and S.A.G. 20 %.

Data availability statement

The data supporting the results of this study is available as ''supplementary files''on the Uniciencia website

References

Ahbirami, R., Zuharah, W. F., Thiagaletchumi, M., Subramaniam, S., & Sundarasekar, J. (2014). Larvicidal efficacy of different plant parts of railway creeper, ipomoea cairica extract against dengue vector mosquitoes, aedes albopictus (Diptera: Culicidae) and aedes aegypti (Diptera: Culicidae). Journal of Insect Science, 14(180). https://doi.org/10.1093/jisesa/ieu042 [ Links ]

Amariles Barrera, S., García Pajón, C., & Parra Henao, G. (2013). Actividad insecticida de extractos vegetales sobre larvas de Aedes aegypti, Diptera: Culicidae. Revista CES Medicina, 27(2), 193-203. https://doi. org/10.21615/cesmedicina [ Links ]

Araya, M., Carvajal, Y., Álvarez, V., Orozco, R., & Rodríguez, G. (2017). Polyphenol characterization of three varieties of Blackberry fruits (Rubus adenotrichos), cultivated in Costa Rica. Journal of Berry Research, 7, 97-107. https://doi.org/10.3233/JBR-170150 [ Links ]

Bisset, J. A., Marin, R., Rodriguez, M. M., Severson, D. W., Ricardo, Y., French, L., Perez, O. (2013). Insecticide resistance in two Aedes aegypti (Diptera: Culicidae) strains from Costa Rica. J Med Entomol, 50(2), 352-361. https://doi.org/10.1603/ME12064 [ Links ]

Boehme, A. K., Noletto, J. A., Haber, W. A., & Setzer, W. N. (2008). Bioactivity and chemical composition of the leaf essential oils of Zanthoxylum rhoifolium and Zanthoxylum setulosum from Monteverde, Costa Rica. Natural Product Research, 22(1), 31-36. https://doi. org/10.1080/14786410601130224 [ Links ]

Calderón, Ó., & Troyo, A. (2016). Evaluación de la resistencia a insecticidas en cepas de Aedes aegypti (Diptera: Culicidae) de la Región Caribe de Costa Rica. Revista Cubana de Medicina Tropical, 68(1), 95-104. [ Links ]

Calderón-Arguedas, Ó., & Troyo, A. (2014). Perfil de resistencia a insecticidas en una cepa de Aedes aegypti (Linnaeus) de la región Caribe de Costa Rica. Revista Cubana de Medicina Tropical, 66(3), 351-359. [ Links ]

Gonzalez, M. (15 de agosto de 2021). Informe de vigilancia de Arbovirus basada en laboratorio: Análisis de datos de virus Zika, dengue, chikungunya, mayaro y fiebre amarilla. https://www.inciensa.sa.cr/vigilancia_epidemiologica/informes_vigilancia/2021/Virologia/Informe%20 Arbovirus%201er%20semestre%202021.pdfLinks ]

Greenop, A., Cook, S. M., Wilby, A., Pywell, R. F., & Woodcock, B. A. (2020). Invertebrate community structure predicts natural pest control resilience to insecticide exposure. Journal of Applied Ecology, 1365-2664.13752. https://doi.org/10.1111/1365-2664.13752 [ Links ]

Gutierrez, O., Arenas, J., Barrera, M., & Martínez, J. (2007). Actividad insecticida de extractos de Bocconia frutescens l. sobre Hypothenemus hampei f. Scientia et Technica, 1(33). [ Links ]

Hasan, H. A., Jaal, Z., Ranson, H., & McCall, P. (2015). Pyrethroid and organophosphate susceptibility status of Aedes aegypti (Linnaeus) and Aedes Albopictus (Skuse) in Penag, Malaysia. International Journal of Entomological Research, 03(03), 91-95. [ Links ]

Haziqah-Rashid, A., Chen, C. D., Lau, K. W., Low, V. L., Sofian-Azirun, M., Suana, I. W., … Azidah, A. A. (2019). Monitoring Insecticide Resistance Profiles of Aedes aegypti (Diptera: Culicidae) in the Sunda Islands of Indonesia Based on Diagnostic Doses of Larvicides. Journal of Medical Entomology, 56(2), 514-518. https://doi.org/10.1093/jme/tjy208 [ Links ]

Jirovetz, L., Buchbauer, G., Stoilova, I., Krastanov, A., Stoyanova, A., & Schmidt, E. (2007). Spice plants: Chemical composition and antioxidant properties of Pimenta Lindl. essential oils, part 1: Pimenta doica (L.) Merr. leaf oil from Jamaica. Ernährung, 31(7/8), 293. [ Links ]

Keziah, E. A., Nukenine, E. N., Yingyang Danga, S. P., & Esimon, C. O. (2016). Synergistic Activity of a Mixture of Lantana camara and Ocimum gratissimum Leaves Extracts against Aedes aegypti Larvae (Diptera: Culicidae). Journal of Mosquito Research, 6(23), 1-10. https://doi.org/10.5376/jmr.2016.06.0023 [ Links ]

Leal, A., de Oliveira, A., Santos, R., Soares, J., Lavor, E., Pontes, M., … Almeida, J. (2020). Determination of phenolic compounds, in vitro antioxidant activity and characterization of secondary metabolites in different parts of Passiflora cincinnata by HPLC-DAD-MS/MS analysis. Natural Product Research, 34(7), 995-1001. https://doi.org/10.1080/14786419.2018.1548445 [ Links ]

Leyva, M., Tacoronte, J., Marquetti, M., Scull, R., Montada, D., Rodríguez, Y., & Bruzón, R. (2008). Actividad insecticida de aceites esenciales de plantas en larvas de Aedes aegypti (Diptera: Culicidae). Revista Cubana de Medicina Tropical, 60(1), 78-82. [ Links ]

Leyva-Silva, M. I., French, L., Pino, O., Montada, D., Morejón, G., & Marquetti, M. del C. (2017). Plantas con actividad insecticida: Una alternativa natural contra mosquitos. Revista Biomédica, 28(3), 139-181. https://doi. org/10.32776/revbiomed.v28i3.571 [ Links ]

Liu, M., Cai, Q. X., Liu, H. Z., Zhang, B. H., Yan, J. P., & Yuan, Z. M. (2002). Chitinolytic activities in Bacillus thuringiensis and their synergistic effects on larvicidal activity. Journal of Applied Microbiology, 93(3), 374-379. https:// doi.org/10.1046/j.1365-2672.2002.01693.x [ Links ]

Lounibos, L. P., & Kramer, L. D. (2016). Invasiveness of Aedes aegypti and Aedes albopictus and vectorial capacity for chikungunya virus. Journal of Infectious Diseases, 214(Suppl 5), S453-S458. https://doi.org/10.1093/infdis/jiw285 [ Links ]

Manjarres-Suarez, A., & Olivero-Verbel, J. (2013). Chemical control of Aedes aegypti: a historical perspective. Revista Costarricense de Salud Pública, 22(1), 68-75. [ Links ]

Mora, S., Castro, V., Chavarría, M., Murillo, R., & Poveda, L. (2011). Chemical constituents from Zanthoxylum setulosum (Rutaceae). Boletin Latinoamericano y Del Caribe de Plantas Medicinales y Aromaticas, 10(2), 155-158. [ Links ]

Muñoz V. J. A.; Staschenko, E.; & Ocampo D., C. B. (2014). Actividad insecticida de aceites esenciales de plantas nativas contra Aedes aegypti (Diptera: Culicidae). Revista Colombiana de Entomologia, 40(2), 198-202. [ Links ]

Olga, O. O., & Braz-Filho, R. (1997). Dibenzylbutyrolactone lignans and coumarins from Ipomoea cairica. Journal of the Brazilian Chemical Society, 8(3), 235-238. https://doi. org/10.1590/s0103-50531997000300009 [ Links ]

Pan American Health Organization. (2019). Dengue. https://www.paho.org/es/temas/dengueLinks ]

Pan American Health Organization. (2020). Dengue National Fever cases https://www.paho.org/data/index.php/en/mnu-topics/indicadores-dengue-en/dengue-nacional-en/252-dengue-pais-ano-en.htmlLinks ]

Paniz-Mondolfi, A. E., Rodríguez-Morales, A. J., Blohm, G., Marquez, M., & Villamil-Gomez, W. E. (2016). ChikDenMaZika Syndrome: The challenge of diagnosing arboviral infections in the midst of concurrent epidemics. Annals of Clinical Microbiology and Antimicrobials, 15(1), 1-4. https://doi.org/10.1186/s12941-016-0157-x [ Links ]

Parra, J., Hernández, P., Ocampo-Maroto, F., Álvarez-Valverde, V., Carvajal-Miranda, Y., Rodríguez-Rodríguez, G., & Herrera, C. (2018). Phytochemical characterization and antioxidant profile of Sechium edule (Jacq) Swartz (Cucurbitaceae) varieties grown in Costa Rica. Journal of Pharmacy and Pharmacognosy Research, 6(6), 448-457. [ Links ]

Patel, K., & Patel, D. K. (2018). Health Benefits of Quassin from Quassia amara: A Comprehensive Review of their Ethnopharmacological Importance, Pharmacology, Phytochemistry and Analytical Aspects. Current Nutrition & Food Science, 16(1), 35-44. https://doi.org/10.2174/1573401314666181023094645 [ Links ]

Patterson, J., Sammon, M., & Garg, M. (2016). Dengue, zika and chikungunya: Emerging arboviruses in the new world. Western Journal of Emergency Medicine, 17(6), 671-679. https://doi.org/10.5811/westjem.2016.9.30904 [ Links ]

Pilon, A. C., del Grande, M., Silvério, M. R. S., Silva, R. R., Albernaz, L. C., Vieira, P. C., … Lopes, N. P. (2022). Combination of GCMS Molecular Networking and Larvicidal Effect against Aedes aegypti for the Discovery of Bioactive Substances in Commercial Essential Oils. Molecules, 27(5). https://doi.org/10.3390/molecules27051588 [ Links ]

Pinto, C. C. C., Menezes, J. E. S. A. De, Melo, D. S., & Feitosa, C. R. S. (2016). Chemical Composition and larvicidal activity against Aedes aegypti of essential oils from Croton jacobinenesis Baill. Boletín Latinoamericano y Del Caribe de Plantas Medicinales y Aromáticas, 15(2), 122-127. [ Links ]

Prabha, I., & Nagarajan, N. (2016). Synthesis, anti-larvicidal and anti-bacterial studies of 7-hydroxy-4methylcoumarin, 7-acetoxy-4-methylcoumarin, 8-acetyl-7-hydroxy-4methylcoumarin, resacetophenone, 8-acetyl-5-hydroxy-4-methylcoumarin and 4, 7-dimethylcoumarin. Research Journal of Pharmacy and Technology, 9(4), 423-429. https://doi.org/10.5958/0974-360X.2016.00078.0 [ Links ]

Rangiah, K., & Gowda, M. (2019). Method to Quantify Plant Secondary Metabolites: Quantification of Neem Metabolites from Leaf, Bark, and Seed Extracts as an Example (pp. 21-30). Springer, Cham. https://doi. org/10.1007/978-3-030-16122-4_3 [ Links ]

Rey, J., & Lounibos, P. (2015). Ecología de Aedes aegypti y Aedes albopictus en América y la transmisión de enfermedades. Biomédica, 35(2), 1-27. https://doi.org/10.7705/biomedica.v35i2.2514 [ Links ]

Setzer, W. N., Stokes, S. L., Bansal, A., Haber, W. A., Caffrey, C. R., Hanseir, E., & McKerrow, J. H. (2007). Chemical composition and cruzain inhibitory activity of croton draco bark essential oil from Monteverde, Costa Rica. Natural Product Communications, 2(6), 685-689. https://doi. org/10.1177/1934578x0700200613 [ Links ]

Silvério, M. R. S., Espindola, L. S., Lopes, N. P., & Vieira, P. C. (2020, August 1). Plant natural products for the control of Aedes aegypti: The main vector of important arboviruses. Molecules. MDPI AG. https://doi.org/10.3390/molecules25153484 [ Links ]

Vargas Miranda, K., Troyo, A., & Calderón Arguedas, Ó. (2019). Resistance of Aedes aegypti Diptera: Culicidae to organophosphate and pyrethroid insecticides in Orotina, Alajuela, Costa Rica. Revista Costarricense de Salud Pública, 28(1), 15-24. [ Links ]

Wang, Z., Kim, J.-R. R., Wang, M., Shu, S., & Ahn, Y.-J. J. (2012). Larvicidal activity of Cnidium monnieri fruit coumarins and structurally related compounds against insecticide-susceptible and insecticide-resistant Culex pipiens pallens and Aedes aegypti. Pest Management Science, 68(7), 1041-1047. https://doi.org/10.1002/ps.3265 [ Links ]

World Health Organization. (1981). Instruction for determining the susceptibility or resistance of adult mosquitoes to organochlorine, organophosphate and carbamate insecticides-diagnostic test. WHO/VBC/81.807:WHO/VBC/81.80. https://apps.who.int/iris/ handle/10665/69615Links ]

World Health Organization. (2004). Global strategic framework for integrated vector management. WHO/CDS/CPE/PVC/2004. https://apps.who.int/iris/handle/10665/68624Links ]

World Health Organization. (2009). Dengue. Guías para el diagnóstico, tratamiento, prevención y control. OMS y Programa Especial Para Investigación y Capacitación de Enfermedades Tropicales. https://iris.paho.org/handle/10665.2/31071Links ]

World Health Organization.(2017).Respuesta mundial para el control de vectores 2017-2030. https://paho.org/es/documentos/respuesta-mundial-para-control-vectores-2017-2030-0Links ]

Received: May 27, 2022; Accepted: February 02, 2023

Creative Commons License This is an open-access article distributed under the terms of the Creative Commons Attribution License