Abstract

ROJAS-VARGAS, Alejandra  and  HINE-GOMEZ, Ana. Micropropagation of Superior Clones of Mahogany (Swietenia macrophylla King) from Nodal Segments. Ciencias Ambientales [online]. 2019, vol.53, n.2, pp.47-59. ISSN 2215-3896.  http://dx.doi.org/10.15359/rca.53-2.3.

Mahogany is the most economically important native forest species in Latin America, it is found in Appendix II of CITES, because it has a high exploitation rate, due to high extraction rates suffered over decades. The objective of this research was to develop a protocol for the in vitro establishment of two clones (33 and 80), belonging to a forest genetic improvement program. Nodal segments of clones 83 and 33 were established under in vitro conditions using 15 % calcium hypochlorite with an exposure time of 20 minutes. The best culture medium for in vitro establishment of the nodes was MS supplemented with 3 % sucrose, 0.016 g / l of agrimicin and benlate, obtaining 55 % of established nodes at 16 days of culture. In both clones, the effect of five concentrations of 6-Benzylaminopurine (6-BAP) (0.0, 0.44, 0.88, 1.33, 1.77, and 2.21 μM L-1) was evaluated sprouting. On average, one outbreak per explant was obtained in the five 1treatments evaluated. No significant statistical differences were observed.

Keywords : Benzylaminopurine; forestry species; genetic improvement program; in vitro culture; nodal segments.

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