SciELO - Scientific Electronic Library Online

 
vol.20 issue3Knowledge of Antibiotic Therapy Protocols by the Students of Costa Rican Dental Schools author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • Have no similar articlesSimilars in SciELO

Share


Odovtos International Journal of Dental Sciences

On-line version ISSN 2215-3411Print version ISSN 1659-1046

Abstract

DELGADILLO AVILA, Juana R.; CAMPODONICO REATEGUI, Carlos H.; GOMEZ MEZA, Dora N.  and  CHACON USCAMAITA, Pamela R.. Presence of Streptococcus Mutans Genotype C in Peruvian Children and Adolescents with Caries. Odovtos [online]. 2018, vol.20, n.3, pp.105-113. ISSN 2215-3411.  http://dx.doi.org/10.15517/ijds.v0i0.34279.

Caries is one of the diseases of infectious nature, chronic transmissible very prevalent in Peru, related to the presence of Streptococcus mutans, hygienic and nutritional habits. Objective: The purpose of this study was to determine the presence of genotype C in Streptococcus mutans in Peruvian children and adolescents, using the PCR-Multiplex technique; and its association with the prevalence of dental caries. Materials and methods: The study was done with a sample of 78 children and adolescents of both sexes from Lima. The study consisted of two phases, on the first one the stimulated saliva was obtained, for the bacterial culture, the same ones that were grown on Mitis Salivarius agar with bacitracin and sulfisoxasol. On the second phase, genotyping was carried out according to its enzymatic profile. For the extraction of DNA, the Gene Extraction Kit GG-1 Bacterial DNA was used, for which cultures of Streptococcus sp strains were performed in the BHI broth with sucrose at 37ºC for 24 hours. Results: The presence of Streptococcus mutans was evidenced in 59 (75.6%) of 78 saliva samples. The results of the genotyping by PCR Multiplex demonstrate the presence of 22 saliva samples of Streptococcus mutans with genotypes C (37,297%) and 37 samples 62, 71 % without this Genotype. Conclusions: The results showed that the presence of genotype C is not related to sex, age group or the presence of dental caries.

Keywords : Streptococcus mutans; Genetics; Saliva; Caries.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )