SciELO - Scientific Electronic Library Online

 
vol.31 número3Dinámica de la severidad de la roya (Hemileia vastatrix) en café, en Chanchamayo (Junín-Perú)Efecto del fertilizante orgánico y mineral en rendimiento de caña panelera en Nariño, Colombia índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Compartir


Agronomía Mesoamericana

versión On-line ISSN 2215-3608versión impresa ISSN 1659-1321

Resumen

ARTAVIA-CARMONA, Roy  y  PERAZA-PADILLA, Walter. Morphological, morphometric and molecular identification of Meloidogyne exigua (Göeldi 1887) in coffee (Coffea arabica). Agron. Mesoam [online]. 2020, vol.31, n.3, pp.530-544. ISSN 2215-3608.  http://dx.doi.org/10.15517/am.v31i3.38733.

Introduction. The genus Meloidogyne contains many species and their correct diagnosis of these must be based on the use of multiple tools, which in diagnostic laboratories allow a correct and reliable evaluation, specially for quarantine nematodes. The proper identification of a phytonematode is important not only to establish an appropriate control strategy, but also to avoid its spread to other areas where it could cause damage that would result in economic losses. Objective. To perform taxonomic and molecular studies to confirm the Meloidogyne species, associated to a coffee plantation located in Barva, Heredia. Materials and methods. Soil and root samples were collected in a coffee plantation located in Barva, Heredia, Costa Rica, which were processed in the Nematology laboratory of the School of Agricultural Sciences at the Universidad Nacional (UNA), using the centrifugation-flotation method in sugary solution and molecular techniques. Results. On average, 30 J2 100 cc-1 were counted in soil and 1000 J2 10 g-1 of roots of the root-knot nematode. Taxonomic and molecular techniques allowed to identify that the females and juveniles extracted from coffee roots corresponded to Meloidogyne exigua. Analysis of the sequences generated by PCR and the use of the DraI enzyme confirmed the identity of the species with 99 % similarity when compared to sequences from Costa Rica and Nicaragua, reported in the Genbank. Conclusion. Using different morphometric and molecular techniques, M. exigua associated with a coffee plantation was identified.

Palabras clave : Coffea arabica; root-knot nematode; identification; molecular analysis.

        · resumen en Español     · texto en Español     · Español ( pdf )