Abstract

PONCIANO-SAMAYOA, Karla Melina  and  HIDALGO-VILLATORO, Sergio Gonzalo. Genetic diversity of roselle in Guatemala  revealed by AFLP markers. Agron. Mesoam [online]. 2012, vol.23, n.1, pp.63-71. ISSN 2215-3608.

The objective of this study was to characterize a collection of 17  genotypes of roselle that were collected in Guatemala. During July 2010/ May 2011, twelve selective combinations of AFLP were amplified at Instituto de Ciencia y Tecnología Agrícolas (ICTA), to identify clusters and to analyze the genetic  diversity within the collection. A 98% of polymorphic loci was observed, nine per amplification in average. Five selective primer pairs were high informative and are recommended for future investigations. No duplicated  genotypes  were  identified. The  clustering and correspondence analysis identified three closely related groups. A fourth group included accession 205, characterized  by early maturity and high yield. The clusters formed were similar to those identified in a previous agromorphological characterization.  Probably, there is a genetic linkage between AFLPs and quantitative trait loci (QTLs) that can be used in marker-assisted selection. The genetic diversity (Nei) was high (0.3053). The genetic differentiation (GST) between groups was 57.62%. The genetic variability within clusters was lower (42.38%) and probably due to  the  low genetic flow (Nm=0.3678) commonly found in inbreed species.

Keywords : Hibiscus   sabdariffa   L.; similarity; clustering; genetic differentiation; genetic flow.

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