Lankesteriana
versão On-line ISSN 1409-3871
Resumo
CRUZ-HIGAREDA, Jesus Bernardo; LUNA-ROSALES, Barbara Susana e BARBA- ALVAREZ, Amadeo. A novel seed baiting technique for the epiphytic orchid rhynchostele cer vantesii, a means to acquire mycorrhizal fungi from protocorms. Lankesteriana [online]. 2015, vol.15, n.1, pp.67-76. ISSN 1409-3871.
We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protocorms in an early stage of development, six of wich were of sufficient size to warrant fungal isolations; Nine strains were isolated in pure culture and were inoculated on seeds and protocorms under in vitro conditions.
Palavras-chave : Orchid; Epiphytic; Seed; Baiting; Mycorrhiza; Rhynchostele.