Abstract

GARRO-MONGE, Giovanni; GARCIA-GOMEZ, David; VALERIN-BERROCAL, Karla  and  JIMENEZ-QUESADA, Karol. Optimization of a metabolic pathway in Escherichia coli for the production of farnesene through the use of synthetic biology. Tecnología en Marcha [online]. 2018, vol.31, n.4, pp.25-36. ISSN 0379-3982.  http://dx.doi.org/10.18845/tm.v31i4.3957.

Interest compounds generation using organic waste as raw material results in an economically and industrially viable option. Through synthetic biology is possible the design of microorganisms capable of using these residues as carbon sources in order to produce biofuels, vaccines, alcohol, oils and other products. Farnesene production and its precursors is possible through the genetic modification of microorganisms like Escherichia coli, introducing metabolic pathways capable of produce the interest compounds. After studying, optimizing and synthesizing the genes that make up the metabolic pathway of mevalonate, which originates farnesene, these were assembled together with several Anderson family promoters to transform E. coli and generate E, E, -farnesene from synthetic form.

Keywords : Sinthetic biology; farnesene; RFC10; mevalonate pathway; terpenes.

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