SciELO - Scientific Electronic Library Online

 
vol.38 issue1Mites associated with the avocado crop (Persea americana Mill) in the Central Coast of Peru author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • Have no similar articlesSimilars in SciELO

Share


Agronomía Costarricense

Print version ISSN 0377-9424

Abstract

GALVIS, Fabián  and  YOLIMA MORENO, Laura. Molecular  characterization  using rep-PCR of native isolates of Bacillus thuringiensis, obtained from soil samples. Agron. Costarricense [online]. 2014, vol.38, n.1, pp.223-229. ISSN 0377-9424.

Bacillus thuringiensis is a Gram-positive bacteria forming spores, which produces parasporal crystals of a proteic nature, toxic against various  orders  of  insects  and  biodegradable and  harmless to other species. This research use the experimental model, allowing, through observation techniques, microbiological and biochemical identification of B thuringiensis from soil samples from municipalities of Cúcuta, El  Zulia, Los Patios, San Cayetano and Villa del Rosario, Norte de Santander, Colombia, and its subsequent characterization with molecular markers Bc-Rep and MB1. Microbiological and biochemical tests identified 10 isolates as B. thuringiensis; the results of phylogenetic analysis showed significant differences in the clusters obtained with Bc-Rep and MB1 markers. With Bc-Rep a low index of similarity (18%) was recorded, while with the marker MB1 a higher similarity index, 58%, was obtained. This work indicated a great genetic variability among isolates, showing that markers Bc-Rep and MB1 are highly effective to differentiate closely related strains, thus becoming a genetic tool of great value for studies of identification and diversity in B. thuringiensis.

Keywords : B. thuringiensis; bioinsecticide; rep-PCR; phylogenetic analysis.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )