Abstract

GARCIA ROJAS, Tatiana  and  ABDELNOUR ESQUIVEL, Ana. Cryopreservation de of shoots and seeds of cedar (Cedrela odorata L.). Agron. Costarricense [online]. 2013, vol.37, n.1, pp.113-126. ISSN 0377-9424.

In order to protect the forest resources of Cedrela odorata L., currently threatened, it was considered necessary to establish a protocol for its cryopreservation. Research was conducted using shoots and seeds. Vitrification techniques were evaluated with shoots, using various solutions and rapid freezing in liquid nitrogen (LN). Initially the best results were  obtained  using  incubation  for  5  min  in PVS3  solution  before  freezing,  survival  being 61% after 3 weeks of culture on Murashige and Skoog (MS) medium with 1.0 mg.l^-1  BAP  and 0.1  mg.l^-1   GA₃.  Moreover,  by  incubating   the apices in 0.3 M sucrose solution for 5 min prior to freezing, the survival rate reached 86% after 2 weeks of culture on MS medium with 1.0 mg.l^-1 BAP, 0.1 mg.l^-1 GA₃  and 0.01 mg.l-1 IAA. When incubation in the solution of 0.3 M sucrose was used  and  recovery  MS  medium  with  1  mg.l^-1 KIN,  0.5  mg.l^-1  GA₃  and  100  mg.l^-1   activated charcoal, the appearance of the surviving shoots improved significantly. For  cryopreservation of cedar  seeds,  the  methodology  of  dehydration and rapid freezing  in liquid nitrogen (LN) was evaluated. Seeds with moisture content between 7.9% and 5.5% were placed in 5 ml polypropylene vials and these were directly immersed in NL. The effect of the storage period in NL (1 h, 24 h, 7 days, 1 month, 3 months and 6 months) on seed  germination  was  evaluated.  There  were no statistical differences in ability of seeds to germinate after storage during different periods.  

Keywords : Cryopreservation; liquid nitrogen; vitrification; in vitro culture; growth regulators; shoots; seeds; cider; Cedrela odorata.

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