Abstract

ESTRADA, Roberto  and  VENEGAS, Patricia. Comparison of different protocols for the cultive of mesenchymal stem cells of adipose origin. Rev. costarric. cienc. méd [online]. 2007, vol.28, n.1-2, pp.21-28. ISSN 0253-2948.

International literature reports different protocols for the isolation and cultive of adipose stem cells (ADSC). Although majority of the isolation protocols are effective, it has been determined that in most of the cases in which these cultive protocols are used, the cell growth rates are poor. Being the stem cells one promising therapeutic option, the group experimented with different cultive media to improve the times of cultive. In this investigation, the cell pellet was planted in a 25 cm2 cultive bottle in four different media: Amniomax Media (GIBCO) (medium 1); Ham’s F10 Media supplemented with 10% SFB, 1% antibiotic and adjusted to 2mm de L-Glutamina (medio 2); Ham’s F10 Media supplemented with 10% SFB, 1% antibiotic and adjusted to 4mm de L-Glutamina (medium 3) and Ham’s F10 supplemented with 20% of autologous serum (SA), 1% antibiotic and adjusted to 4mm de L-Glutamina (medium 4). This experiment determines that medium 1 stimulates faster growth rates than the others, obtaining confluent levels in 9 days (approximately 6 million ADSC).

Keywords : mesenchymal stem cells; adipose tissue.

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