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vol.21 issue3-4Detección del efecto citotóxico de la alfa hemolisina de E. coli (Hly A) en leucocitos polimorfonucleares neutrófilos mediante citometría de flujoAccidente y cuadro clínico por la "picadura" de alacranes en Costa Rica author indexsubject indexarticles search
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Revista Costarricense de Ciencias Médicas

Print version ISSN 0253-2948

Abstract

GARCIA, Jorge. La alfa hemolisina de Escherichia coli potencia el metabolismo oxidativo de neutrófilos humanos en respuesta al péptido quimiotáctico FMLP: comparación con el ionóforo de calcio A23187. Rev. costarric. cienc. méd [online]. 2000, vol.21, n.3-4, pp.141-159. ISSN 0253-2948.

The calcium ionophore ionomycin primes polymorphonuclear leukocytes (PMN) for increased superoxide production upon stimulation with the chemotactic peptide FMLP (Helman Finkel, T. et al. J Biol Chem 1987; 262: 12589-12596). In this investigation we assessed the effect of PMN priming with either alpha hemolysin (AH) or the calcium ionophore A23187, both of which increase intracellular calcium, on the oxidative metabolism of PMN (as measured by chemiluminescence) in response to secondary stimulation with FMLP. Both A23187 and AH priming increased the luminol-enhanced chemiluminescence in response to secondary stimulation with FMLP, indicating overstimulation of PMN oxidative metabolism. Additional experiments using lucigenin as chemiluminescence enhancer showed that A23187, but not AH priming of PMN, increased superoxide release in a manner similar to that reported for ionomycin. These results are discussed in reference to infectious processes involving hemolytic E. coli.

Keywords : polymorphonuclear leukocytes; chemiluminescence; luminol; lucigenin; A23187; alpha hemolysin; FMLP.

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