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Revista de Biología Tropical
On-line version ISSN 0034-7744Print version ISSN 0034-7744
Abstract
VASQUEZ, Viviana; MONTERO-ASTUA, Mauricio and RIVERA, Carmen. Incidencia y distribución altitudinal de 13 virus en cultivos de Solanum tuberosum (Solanaceae) en Costa Rica. Rev. biol. trop [online]. 2006, vol.54, n.4, pp.1135-1141. ISSN 0034-7744.
Incidence and altitudinal distribution of 13 virus cultures in Solanum tuberosum (Solanaceae) from Costa Rica. A survey was conducted in 30 fields located at three different altitudes in Cartago, Costa Ricas main potato producing area. Twenty plants were sampled per farm, for a total of 600 samples with 200 samples per altitude. ELISA was used with commercial reagents to independently test for PVX, PVY, PVM, PVA, PVS, PLRV, PMTV, PAMV, PVV, PVT, APLV, APMoV and TRSV. The presence of the following viruses was determined: PVX (77 %), PAMV (62 %), PLRV (42 %), TRSV (42 %), PVT (39 %), PVV (37 %), PMTV (31 %), PVY (30 %), PVS (19 %), PVM (13 %), PVA (8 %), and APMoV (8 %). APLV was not detected in any sample. This is the first report in Costa Rica of the presence of the viruses PMTV, PAMV, PVV, PVT and APMoV. A high viral incidence in the tuber seed production area as well as a high rate of mixed infections is reported. Rev. Biol. Trop. 54 (4): 1135-1141. Epub 2006 Dec. 29
Keywords : potato viruses; diagnosis; DAS ELISA; TAS ELISA; epidemiology; seed potato.