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Revista de Biología Tropical
On-line version ISSN 0034-7744Print version ISSN 0034-7744
Abstract
RIVERA, Carmen and MOREIRA CARMONA, Lisela. Inclusion bodies induced by bean rugose mosaic virus seen under light microscopy. Rev. biol. trop [online]. 2000, vol.48, n.4, pp.955-959. ISSN 0034-7744.
Two types of inclusion bodies were consistently observed under light microscopy in bean (Phaseolus vulgaris) leaf tissue infected with bean rugose mosaic virus (BRMV), a species of the genus Comovirus, family Comoviridae. One type consisted of vacuolated inclusions found mainly in the cytoplasm of epidermal cells. The other type consisted of abundant crystalloid inclusions of different sizes and shapes found consistently in glandular hairs, guard cells, phloem tissue, xylem elements and occasionally in epidermal and mesophyll tissues. The two types of inclusion bodies stained with Azure A and Luxol Brilliant Green Bl-Calcomine Orange 2RS (O-G), and were similar to those seen to be caused by other species of comoviruses.
Keywords : Comovirus; cytopathology; light microscopy; diagnosis; bean.