Resumo

ROJAS-CORDERO, Elizabeth. Quantification of viral hepatitis type B using the real-time PCR technique. Acta méd. costarric [online]. 2008, vol.50, suppl.3, pp.13-15. ISSN 0001-6002.

The polymerase chain reaction technique (PCR) has determined a special advance in the virus quantification for the management of chronic viral infections, especially for HIV hepatitis virus type B and C. The real-time quantification is performed in the ABI PRISM Sequence Detection System. The fragment pb of the genome of the hepatitis type B virus is amplified. The HBV PCR kit is recommended for taking a sample and a protocol for storing it is determined. It is important to know that freezing the samples or keeping them for a long time decreases the sensitivity of the method. It may be stored for years if kept at a temperature of -70° C. Heparinized patients or tubes with heparin will alter the determination of the sample. The lower limit of detection of B virus is 3.78 Ul/ml and the higher limit is 1.4 x 10¹¹ Ul/ml. All genotypes from A-H are determined. The patients with HbeAg positive usually present higher values than 1 x 10⁶ Ul/ml and the HBe negative inactive carriers usually get lower values than 1 x 10⁴.

Palavras-chave : HBV PCR; ABI PRISM 700 Sequence Detection System.

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