Resumo

AGUILAR-ULLOA, Wendy; ARCE-ACUNA, Priscilla; GALIANO-MURILLO, Fiorella  e  TORRES-CRUZ, Terry J.. Spore isolation and evaluation of inoculation methods in the production of mycorrhizae in trap crops. Tecnología en Marcha [online]. 2016, vol.29, suppl.3, pp.5-14. ISSN 0379-3982.  http://dx.doi.org/10.18845/tm.v29i7.2700.

Soil microorganisms play an important role in agriculture, among them we find mycorrhizae, which generate to the plant a great quantity of benefits. In this investigation we took soil and root samples from bean plants. Spores were isolated by the method of wet sieving and decanting. We did a spore count and stained roots and then determined the percentage of AMF colonization. We inoculated 15 pots with each treatment; spores, soil and roots, and we planted bean seeds on each one of them. A month later we measured the length of the roots and stems of every treatment. Also, to determine the inoculum development we determined the quantity of spores produced and the percentage of AMF colonization of the roots. Roots and stems length of the plants evaluated did not present statistically significant differences among the different treatments. The spore inoculation method presented the higher mycorrhization percentage. The methods of inoculation that favored the production of reproductive structures were the treatments with isolated spores and roots; also both of them presented the higher quantity of germinated spores. We recommend for use in crops like beans to use root inoculum from infected plants.

Palavras-chave : Mycorrhiza; symbiosis; Phaseolus vulgaris.

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